Carrier for immobilizing protein and preparation method therefor
AssigneeSouth China University of Technology
InventorsZhanglin LIN, Yanrui YE, Xiaofeng YANG, Qiao LIN
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Abstract
A SpyCatcher peptide-modified carrier, a protein immobilizing carrier, and a protein immobilizing method. The method comprises: linking a SpyCatcher peptide to a carrier so as to obtain a SpyCatcher peptide-modified carrier; and then based on a SpyCatcher-SpyTag reaction, immobilizing a target protein containing SpyTag to the SpyCatcher-modified carrier. A protein immobilization process in which the method is used is fast, a condition is mild, no extra chemical reagent is needed, the immobilization efficiency and an activity recovery rate are high, and the homogeneity is good.
Claims19
A carrier modified by a SpyCatcher peptide, wherein the carrier, when not modified by the SpyCatcher peptide, comprises a group capable of reacting with an amino group (NH2), wherein the SpyCatcher peptide is covalently attached to the carrier through reaction of an amino group with the group, and the SpyCatcher peptide linked to the carrier is capable of forming an isopeptide bond with a SpyTag peptide, preferably the group is selected from the group consisting of an epoxy group, an aldehyde group, an imide, a cyanate, an imidocarbonate and a hydrazide group.
An epoxy resin carrier modified by a SpyCatcher peptide, wherein the SpyCatcher peptide is covalently attached to the carrier through reaction of an amino group with an epoxy group, preferably the epoxy resin carrier is made of a polyacrylate.
A method of preparing a carrier modified by a SpyCatcher peptide, comprising:
- (1) providing a carrier comprising a group capable of reacting with an amino group (NH2), the group being preferably selected from the group consisting of an epoxy group, an aldehyde group, an imide, a cyanate, an imidocarbonate and a hydrazide group;
- (2) bringing a SpyCatcher peptide into contact with the carrier in a liquid environment;
- optionally, activating the group before contact, and/or blocking the carrier after contact;
- and
- (3) optionally, isolating the carrier modified by the SpyCatcher peptide.
Dependencies are inferred from the claim text.