Method for producing reassortant reoviridae virus, and vector library for same
AssigneeSK Bioscience Co., Ltd.
InventorsKi Weon SEO, Tae Woo KWON, Seo Yeon JUNG, Min Jung PARK, Kun Se LEE, Hyun Joo LEE
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Abstract
The present application relates to a method for producing a reassortant Reoviridae virus and a vector library for the same, and provides: a method for producing a reassortant Reoviridae virus by using a cell line into which an RNA polymerase is introduced and an expression vector library according to an aspect; a reassortant Reoviridae virus produced by the method; and an expression vector library for producing reassortant rotavirus.
Claims24
A method for producing a reassortant Reoviridae virus, the method comprising:
- transfecting a first cell line, into which an RNA polymerase gene is introduced, into an expression vector library comprising a foreign gene;
- culturing a first culture by culturing, in a growth medium, the first cell line undergoing the transfection;
- culturing a second culture by adding a second cell line to the first culture that has been cultured;
- and
- culturing a third culture by adding trypsin to the second culture that has been cultured,
- wherein the expression vector library comprises a gene segment of cDNA of the Reoviridae virus and a promoter capable of binding to the RNA polymerase.
An expression vector library for producing reassortant rotavirus, the expression vector library comprising a T7 promoter capable of binding to a T7 RNA polymerase and a plurality of expression vectors each comprising a gene segment of rotavirus cDNA operably linked to the T7 promoter,
- wherein the plurality of expression vectors comprise a gene segment of cDNA of one of VP1, VP2, VP3, VP4, VP6, VP7, NSP1, NSP2, NSP3, NSP4, and NSP5.
Dependencies are inferred from the claim text.