Improved fiber-wash in corn wet-milling
AssigneeNovozymes A/S
InventorsSvend Gunnar Kaasgaard, Mary Ann Stringer, Madelyn Mallison Shoup, Bemardo Vidal, Wei Peng, Xiangyu Cai, Yi Cao
Life of this patent
Estimating the term…
Abstract
The instant application provides methods to increase the total starch yield and/or gluten yield from corn kernels in a wet milling process, the method comprising admixing corn kernels or a fraction of the corn kernels with a Protein Disulfide Isomerase or Thioredoxin.
Claims21
1-73. (canceled)
A method for increasing starch yield and/or gluten yield from corn kernels in a wet milling process, the method comprising contacting ground a fiber rich fraction of ground corn kernels with an effective amount of a polypeptide that catalyzes changes in protein disulfide bonds selected from the group consisting of oxidation, reduction, and isomerization.
An isolated or purified polypeptide having protein disulfide isomerase activity, selected from the group consisting of:
- (a) a polypeptide having at least 85% sequence identity to SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4;
- (b) a polypeptide having at least 85% sequence identity to amino acids 21-516 of SEQ ID NO:
- 1, amino acids 21-513 of SEQ ID NO:
- 2, amino acids 21-516 of SEQ ID NO:
- 3, or amino acids 21-517 of SEQ ID NO:
- 4;
- (c) a polypeptide having at least 85% sequence identity to a mature polypeptide of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4;
- (d) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4;
- (e) a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide encoding sequence of SEQ ID NO:
- 20, SEQ ID NO 21, SEQ ID NO:
- 22, or SEQ ID NO:
- 23;
- and
- (f) a fragment of the polypeptide of (a), (b), (c), (d), or (e) that has protein disulfide isomerase activity.
An isolated or purified thioredoxin polypeptide having the ability to reduce protein disulfide bonds, selected from the group consisting of:
- (a) a polypeptide having at least 85% sequence identity to SEQ ID NO:
- 5;
- (b) a polypeptide having at least 85% sequence identity to amino acids 1-110 of SEQ ID NO:
- 5;
- (c) a polypeptide having at least 85% sequence identity to a mature polypeptide of SEQ ID NO:
- 5;
- (d) a polypeptide derived from a mature polypeptide of SEQ ID NO:
- 5 by substitution, deletion or addition of one or several amino acids in the mature polypeptide of SEQ ID NO:
- 5;
- (e) a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide encoding sequence of SEQ ID NO:
- 24;
- and
- (f) a fragment of the polypeptide of (a), (b), (c), (d), or (e) that has thioredoxin activity activity.
An isolated or purified polynucleotide encoding the polypeptide of 88.
Dependencies are inferred from the claim text.