Novel method for preparing poly(3-hydroxybutyrate-co-hydroxybutyrate)
AssigneeCj Cheiljedang Corporation
InventorsYae Seul PARK, Hyang CHOI, Dong-Eun CHANG, Ji Sun LEE, Imsang LEE
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Abstract
The present disclosure relates to a novel method for preparing poly(3-hydroxybutyrate-co-4-hydroxybutyrate), a microorganism using the biosynthetic pathway of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) of the present disclosure, a composition for producing poly(3-hydroxybutyrate-co-4-hydroxybutyrate), and a method for regulating the 4-hydroxybutyrate content of poly(3-hydroxybutyrate-co-4-hydroxybutyrate).
Claims21
A method for preparing poly(3-hydroxybutyrate-co-4-hydroxybutyrate), comprising:
- (a) converting crotonyl-CoA into 3-hydroxybutyryl-CoA in the presence of enoyl-CoA hydratase and converting crotonyl-CoA into 4-hydroxybutyryl-CoA in the presence of 4-hydroxybutyryl-CoA dehydratase;
- and
- (b) preparing poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by polymerization of 3-hydroxybutyryl-CoA and 4-hydroxybutyryl-CoA in the presence of PHA synthase.
The method according to claim 14, wherein the microorganism comprises a gene encoding enoyl-CoA hydratase, a gene encoding 4-hydroxybutyryl-CoA dehydratase, and a gene encoding PHA synthase.
10-12. (canceled)
Dependencies are inferred from the claim text.