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US 2024/0301439 A1Published application
Downy mildew resistant lettuce mutant
AssigneeNunhems B.V.
InventorWim VRIEZEN
Life of this patent
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Abstract
The invention relates to lettuce plants comprising in its genome a mutant homoserine kinase allele in homozygous form, whereby the plants are resistant against Bremia lactucae.
Claims17
A cultivated plant of the species Lactuca sativa which is homozygous for a mutant allele of the wild type homoserine kinase gene encoding SEQ ID NO:
- 1, wherein the mutant allele a) encodes a mutant homoserine kinase protein comprising one or more amino acids inserted, deleted or replaced compared to the wild type protein of SEQ ID NO:
- 1, or b) has reduced gene expression compared to the wild type allele, wherein said mutant allele results in the leaf tissue of said lettuce plant accumulating L-homoserine, while a plant heterozygous for the mutant allele or lacking the mutant allele does not accumulate L-homoserine in the leaf tissue and wherein said L-homoserine in the leaf tissue confers resistance against Bremia lactuca.
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A method for generating and/or selecting a lettuce plant comprising at least 10.0 nmol L-homoserine per mg fresh weight of leaf tissue, comprising:
- a) providing a plurality of lettuce plants which are homozygous for a mutant allele of a homoserine kinase gene, which mutant allele encodes a mutant homoserine kinase protein comprising one or more amino acid insertions, deletions or substitutions compared to the wild type protein of SEQ ID NO:
- 1, or which mutant allele has reduced gene expression compared to the wild type allele,
- b) analyzing the amount of L-homoserine produced in the leaf tissue of the plants, and
- c) identifying, and optionally selecting, a plant which comprises in its leaf tissue at least 10.0 nmol L-homoserine per mg fresh weight of leaf tissue.
1 more element
A method for detecting whether a lettuce plant or plant part comprises a mutant homoserine kinase allele encoding a protein of SEQ ID NO:
- 1 which comprises one or more amino acid insertions, deletions or substitutions comprises
- a) determining the presence in the plant of a mutant nucleic acid molecule comprising one or more nucleotides which are different compared to the genomic DNA of SEQ ID NO:
- 2 or the mRNA of SEQ ID NO:
- 2 and whereby the encoded protein comprises one or more amino acids which are different from SEQ ID NO:
- 1 or which are inserted or deleted compared to SEQ ID NO:
- 1;
- or
- b) determining the presence in the plant of a protein comprising one or more amino acids which are different from the protein of SEQ ID NO:
- 1 or which are inserted or deleted compared to SEQ ID NO:
- 1.
7 more elements
Dependencies are inferred from the claim text.