Polynucleotide and expression cassette comprising coding sequence of toluene dioxygenase, and use thereof
AssigneesShanghai Shengping Medical Equipment Co., Ltd., Shanghai VastPro Technology Development Co., Ltd., Zhejiang Boxiao Biopharmaceutical Co., Ltd.
InventorsYong YANG, Ping HUANG, Biao JIANG, Wenfeng ZHU
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Abstract
The present disclosure relates to the field of bioengineering and pharmaceutical and chemical production. In particular, provided is a polynucleotide comprising a coding sequence of toluene dioxygenase. Also provided are an expression cassette, a vector and a host cell comprising the polynucleotide, as well as use thereof in the preparation of cis-cyclohexadiene o-diol compounds.
Claims22
A polynucleotide, comprising coding sequences of toluene dioxygenase TodC1, TodC2, TodB and TodA, wherein the polynucleotide comprises, from 5′-end to 3′-end:
- (1) a first nucleotide sequence comprising the coding sequence of TodC1, wherein the TodC1 has an amino acid sequence of SEQ ID NO:
- 4;
- (2) a second nucleotide sequence comprising the coding sequence of TodC2, wherein the TodC2 has an amino acid sequence of SEQ ID NO:
- 5;
- (3) a third nucleotide sequence comprising the coding sequence of TodB, wherein the TodB has an amino acid sequence of SEQ ID NO:
- 6;
- and
- (4) a fourth nucleotide sequence comprising the coding sequence of TodA, wherein the TodA has an amino acid sequence of SEQ ID NO:
- 7;
- wherein the first, second, third and fourth nucleotide sequences each independently use E. coli preferred codons, and the stop codon TAA for TodB and the start codon ATG for TodA form a nested sequence TAATG.
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An expression cassette or vector, comprising a polynucleotide comprising a nucleotide sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.