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US 2024/0301368 A1Published application
Versatile peroxidases and uses thereof
AssigneeYeda Research and Development Co. Ltd.
InventorsSarel FLEISHMAN, Shiran BARBER-ZUCKER
Life of this patent
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Abstract
Recombinant peroxidase enzymes are disclosed having mutations that increase yield when expressed in yeast cells as compared to their corresponding wild-type peroxidase enzyme. Methods of generating are also disclosed as well as uses thereof.
Claims20
A peroxidase enzyme comprising:
- (i) an amino acid sequence at least 85% identical to SEQ ID NO:
- 1;
- and
- (ii) mutations set forth in A11S, V15T, A17S, A19E, P26D, D38G, A40Q, D44Q, T79I, V81T, E91D, P98S, A101Q, G115A, K138T, A140P, I143P, T152S, I153V, D161A, V166S, S167P, E187P, I205F, I214T, S215T, H223P, Q227K, K231P, D246P, S249A, T256V, S267R, A282D, A294P, A295P, V297T, G318A, A319P, A322T, G324P, V330P and R335P, wherein the coordinates correspond to said SEQ ID NO:
- 1;
- wherein the peroxidase enzyme is characterized by increased yield when expressed in yeast cells as compared to wild-type peroxidase enzyme having an amino acid sequence as set forth in SEQ ID NO:
- 4.
4 more elements
A peroxidase enzyme comprising:
- (i) an amino acid sequence at least 85% identical to SEQ ID NO:
- 2;
- and
- (ii) mutations set forth in R4P, M5S, E34T, G59A, T65W, A67Q, V85I, V95I, P104H, I107Q, T125V, S135P, S141P, D143P, S161Q, S164A, S167A, D172S, D229Q, A247L, S249A, S252P, R265Q, A266D, L269Q, A270S, E273A, G274A, A288S, T290I, A299P, T300P, S323D, A325P, S330P, A333P, A335P and A341P, wherein the coordinates correspond to said SEQ ID NO:
- 2;
- wherein the peroxidase enzyme is characterized by increased yield when expressed in yeast cells as compared to wild-type peroxidase enzyme having an amino acid sequence as set forth in SEQ ID NO:
- 5.
4 more elements
A peroxidase enzyme comprising:
- (i) an amino acid sequence at least 85% identical to SEQ ID NO:
- 3;
- and
- (ii) mutations set forth in A11S, V15T, A17S, P26D, I27L, L28R, E29D, E40Q, T74I, P78T, V80L, A100Q, K137T, A139P, I142D, T151S, T153D, D157A, G160A, A165S, A166P, V306T, A321T, A323P and A331S, wherein the coordinates correspond to said SEQ ID NO:
- 3;
- wherein the peroxidase enzyme is characterized by increased yield when expressed in yeast cells as compared to wild-type peroxidase enzyme having an amino acid sequence as set forth in SEQ ID NO:
- 6.
4 more elements
Dependencies are inferred from the claim text.