Polymer dye modification and applications

A conjugated polymer according to Formula (I):

• wherein:
• each A is independently selected from the group consisting of an aromatic co-monomer and a heteroaromatic co-monomer;
• each L1 is a linker moiety independently selected from the group consisting of a cyclic group, a trivalent arylalkyl moiety, and a chain of between 2 and 100 backbone carbon atoms in length, between 2 and 50 backbone carbon atoms in length, or between 2 and 20 backbone carbon atoms in length, wherein each linker moiety can independently be substituted or unsubstituted, saturated or unsaturated and optionally have one, two, three, four or five carbon atoms of the linker backbone optionally replaced with one or more of sulfur, nitrogen, oxygen, B, Al, Si, P, or Se;
• L2 and L3 are each independently linker moieties;
• each W is independently a water-solubilizing moiety;
• each E is an independently selected chromophore, functional moiety, or binding agent;
• each B is independently selected from the group consisting of an aromatic co-monomer, a heteroaromatic co-monomer, a bandgap-modifying monomer, optionally substituted ethylene, and ethynylene;
• G1 and G2 are each independently selected from an unmodified polymer terminus and a modified polymer terminus, each optionally substituted with a binding agent or substrate;
• subscripts n and m are independently integers ranging from 1 to 10,000,
• subscript p is an integer ranging from 0 to 10,000, and
• the sum of subscripts n, m, and p ranges from 2 to 10,000;
• subscript q is 1, 2, 3, or 4;
• subscript r is 1, 2, 3, or 4;
• subscript s is 0, 1, 2, or 3;
• subscript t is 1 or 2
• the sum of subscript r and s ranges from 1 to 4;
• and
• A and B are distributed randomly or non-randomly in the conjugated polymer.

A method of making a conjugated polymer according to Formula II:

• the method comprising converting a conjugated polymer according to Formula IIa:
• to the polymer according to Formula II, wherein:
• each A is independently selected from the group consisting of an aromatic co-monomer and a heteroaromatic co-monomer;
• L1a and L2 are each independently linker moieties;
• each W is independently a water-solubilizing moiety;
• each E is an independently selected chromophore, functional moiety, or binding agent;
• each B is independently selected from the group consisting of an aromatic co-monomer, a heteroaromatic co-monomer, a bandgap-modifying monomer, optionally substituted ethylene, and ethynylene;
• G1 and G2 are each independently selected from an unmodified polymer terminus and a modified polymer terminus;
• each R1 is independently selected from the group consisting of H and an amine protecting group;
• subscripts n and m are independently integers ranging from 1 to 10,000,
• subscript p is an integer ranging from 0 to 10,000, and
• the sum of subscripts n, m, and p ranges from 2 to 10,000;
• subscript q is 1, 2, 3, or 4;
• subscript r is 1, 2, 3, or 4;
• subscript s is 0, 1, 2, or 3;
• subscript t is 1 or 2;
• the sum of subscript r and s ranges from 1 to 4;
• and
• A and B are distributed randomly or non-randomly in the fluorescent polymer.