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AssigneeGenomatica, Inc.
InventorsAmit Mahendra SHAH, Deqiang ZHANG, Joseph Roy WARNER, Benjamin Matthew GRIFFIN, Jinel SHAH, Justin Robert COLQUITT
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The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme. The invention provides a non-naturally occurring microbial organism that has a 1,6-hexanediol (HDO) pathway with a HDO pathway enzyme expressed in sufficient amounts to produce 6 aminocaproate semialdehyde, HDO, or both. The invention further provides a non-naturally occurring microbial organism that has an HMD pathway with a HMD pathway enzyme expressed in sufficient amounts to produce 6-aminocaproate semialdehyde, HMD, or both. The invention additionally provides bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO and methods for producing bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO.
An engineered carboxylic acid reductase (CAR) enzyme comprising at least one alteration of one or more amino acids of Variant 1 of Table 14 at one or more residue positions comprising A180, A234, A259, A420, L424, M296, M412, N401, Q430, T489, V403, V423, and combinations thereof.
An engineered transaminase (TA) enzyme comprising at least one alteration of one or more amino acids of Variant 1 of Table 13 at one or more residue positions comprising A13, A298, A325, C388, I49, K155, L334, Q375, R410, S287, V386, V79, and combinations thereof.
An engineered a hexamethylenediamine (HMD) transaminase enzyme (TA2) comprising at least one alteration of one or more amino acids of the sequence one of one of SEQ ID NOS:265, and 267-296 at one or more residue positions comprising A10, C297, E120, F327, F91, I240, I309, L11, L327, L419, L4, N2, P326, Q119, R426, S153, T191, T275, T330, and combinations thereof.
The non-naturally occurring microbial organism of any one of claims 47-52, comprising a CAR having an amino acid sequence selected from the group consisting of CAR Variants 2-53 as set forth in Table 14, or CAR Variants 56-79 as set forth in Table 15.
A culture medium comprising bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO, wherein said bioderived HMD, 6-aminocaproate semialdehyde, and/or HDO has a carbon-12, carbon-13 and carbon-14 isotope ratio that reflects an atmospheric carbon dioxide uptake source.
Bioderived AMD, 6-aminocaproate semialdehyde, and/or HDO having a carbon-12, carbon-13 and carbon-14 isotope ratio that reflects an atmospheric carbon dioxide uptake source.
Dependencies are inferred from the claim text.