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US 2024/0294876 A1Published application
Methods for the expansion of human granulocyte-macrophage progenitors and applications thereof
AssigneeUniversity of Southern California
InventorsQi-Long Ying, Zheng Guo, Shi Yue, Tai Nguyen, Jiaqi Tang, Chao Zhang
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Abstract
The disclosure provides methods for the long-term expansion of granulocyte-macrophage progenitors, the granulocyte-macrophage progenitors generated therefrom, and uses of the granulocyte-macrophage progenitors thereof.
Claims30
A method for the expansion of a population of human granulocyte/macrophage progenitor cells (GMPs), comprising:
- culturing GMPs in a culture medium comprising:
- (i) a growth factor;
- (ii) a B-Raf kinase inhibitor;
- (iii) an agent that inhibits the mitogen-activated kinase interacting protein kinases 1 and 2 (Mnk1/2);
- (iv) an agent that inhibits the PI3K pathway;
- (v) a compound having the structure of Formula I:
- wherein,
- R1 is selected from:
- R2 is selected from:
- R3 is selected from:
- n is an integer selected from 0, 1, 2, 3, 4, and 5;
- wherein the GMPs maintain their morphological characteristics after undergoing multiple cell passages and/or clonal expansion.
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A compound having the structure of Formula I:
- wherein,
- R1 is selected from:
- R2 is selected from:
- R3 is selected from:
- n is an integer selected from 0, 1, 2, 3, 4, and 5. In a further embodiment, a compound having the structure of Formula I is not
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A cell culture medium comprising a compound having the structure of Formula I:
- wherein,
- R1 is selected from:
- R2 is selected from:
- R3 is selected from:
- n is an integer selected from 0, 1, 2, 3, 4, and 5. In a further embodiment, a compound having the structure of Formula I is not
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Dependencies are inferred from the claim text.