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US 2024/0292858 A1Published application
Method for producing a coffee extract
AssigneeNovozymes A/S
InventorsJens Magnus EKLOF, Louise Rasmussen, Gitte Budolfsen Lynglev`, Nikolaj Spodsberg, Kristian Bertel Roemer Krogh
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Abstract
The present invention relates to a method for producing a coffee extract which comprises use of an enzyme having mannanase activity. The invention also relates to polypeptides having endo-beta-1,4-mannanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims21
1-15. (canceled)
A method for producing a coffee extract, comprising:
- (a) incubating roasted and ground coffee beans in water with a GH5 endo-beta-1,4-mannanase, at a temperature of 50-80° C. to make an aqueous coffee extract, wherein the GH5 endo-beta-1,4-mannanase comprises an amino acid sequence with at least 90% sequence identity to SEQ ID NO:
- 3, has mannanase activity, and is thermostable;
- and
- (b) separating the coffee extract from the coffee beans.
1 more element
Dependencies are inferred from the claim text.