Anti-crispr construct and its use to counteract a crispr-based gene-drive in an arthropod population
AssigneesImperial College Innovations Limited, Polo D’Innovazione di Genomica Genetica e Biologia SRL
InventorsChrysanthi Taxiarchi, Roberto Galizi, Andrea Crisanti, Alekos Simoni, Rocco D'Amato
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Abstract
The present invention relates to an anti-CRISPR construct useful to counteract the spread of a gene-drive in an arthropod population. The invention is also concerned with a system comprising the anti-CRISPR construct and a crispr-based gene-drive construct, a method of producing a genetically modified arthropod, a genetically modified arthropod, and a method for counteracting a CRISPR-based gene-drive in an arthropod population.
Claims27
An anti-CRISPR construct comprising a germline specific promoter sequence operably linked to a nucleotide sequence coding for an Acr protein.
The construct according to any one of the preceding claims, wherein the promoter sequence is a promoter sequence that substantially restricts expression of the nucleotide sequence to germline cells of an arthropod.
The construct according to any one of the preceding claims, wherein the construct further comprises attB or attP integrase attachment sites which, respectively, flank the nucleotide sequence coding for the Acr protein or the NLS-tagged Acr protein, and the promoter sequence.
The construct according to any one of the preceding claims, wherein the construct further comprises piggyBac transposon terminal repeats, which, respectively, flank the nucleotide sequence coding for the Acr protein or the NLS-tagged Acr protein, and the promoter sequence.
The construct according to any one of the preceding claims, wherein the construct comprises or consists of a nucleic acid sequence substantially as set out in SEQ ID NO:
- 20, or a fragment or variant thereof.
The construct according to any one of the preceding claims, wherein the construct is inserted within a nucleic acid sequence comprising or consisting of the nucleotide sequence substantially as set out in SEQ ID NO:21, or a fragment or variant thereof.
The construct according to any one of the preceding claims, wherein the construct is inserted at the TTAA site of SEQ ID NO:22, or a fragment or variant thereof.
A method of producing a genetically modified arthropod, the method comprising introducing into an arthropod an anti-CRISPR construct comprising a nucleotide sequence encoding an Acr protein.
A genetically modified arthropod comprising an anti-CRISPR construct comprising a nucleotide sequence encoding an Acr protein.
Dependencies are inferred from the claim text.