US 2024/0280575 A1Published application

Compositions and Methods for the Detection of Mycoplasma

AssigneeIDEXX Laboratories, Inc.

InventorsRaghavan PALANIAPPAN, Ethan Everett Pope, Maha Yerramilli

Life of this patent

Estimating the term…

Abstract

Provided herein are compositions and methods for detection of detection and treatment of Mycoplasma haemofelis and Mycoplasma haemocanis.

Claims33

Dependent claims

One or more polypeptides comprising one or more of:

(i) a polypeptide as set forth in SEQ ID NO:1, 2, 3, 4, 5, 6, 7, 8, 37, 54, 74, 97, 111, 140, 141, or 151;
(ii) a polypeptide comprising SEQ ID NO:1, wherein:
(a) Wat position 11 is retained and 1, 2, or 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, or 15;
(c) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
2, 3, 4, 6, 7, 8, or 14;
or
(d) W at position 11 is retained and position 12 is substituted with A, and optionally conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 13, 14, or 15;
(iii) a polypeptide comprising SEQ ID NO:2, wherein:
(a) W at position 11 is retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, or 15;
or
(c) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
2, 3, 4, 6, 7, 8, or 14;
(iv) a polypeptide comprising SEQ ID NO:3 wherein:
(a) W at position 10 is retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 10 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, or 15;
or
(c) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 3, 5, 6, 7, 8, 14 or 15;
(v) a polypeptide comprising SEQ ID NO:4, wherein:
(a) W at position 10 is retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 10 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, or 15;
or
(c) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 3, 5, 6, 7, 8, 14 or 15;
(vi) a polypeptide comprising SEQ ID NO:5, wherein:
(a) K at position 9 and Wat position 13 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) K at position 9 and W at position 13 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 14, or 15;
or
(c) K at position 9 and W at position 13 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
2, 3, 4, or 5;
(vii) a polypeptide comprising SEQ ID NO:6, wherein:
(a) K at position 9 and the W at position 13 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) K at position 9 and W at position 13 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 14, or 15;
or
(c) K at position 9 and W at position 13 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
2, 3, 4, or 5;
(viii) a polypeptide comprising SEQ ID NO:7, wherein:
(a) K at position 7, D at position 8, and W at position 9 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) K at position 7, D at position 8, and W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 10, 11, 12, 13, 14, or 15;
or
(c) K at position 7, D at position 8, and W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
3, 4, or 15.
(ix) a polypeptide comprising SEQ ID NO:8, wherein
(a) D at position 8 and W at position 9 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) D at position 8 and W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 10, 11, 12, 13, 14, or 15;
or
(c) D at position 8 and the W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
3, 4, 7, or 15;
(x) a polypeptide comprising SEQ ID NO:10;
(xi) a polypeptide having 95% or more sequence identity to SEQ ID NO:
10, wherein amino acids 75-89, 170-184, or 75-89, and 170-184 are retained;
(xii) a polypeptide comprising SEQ ID NO:
12;
(xiii) a polypeptide having 95% or more sequence identity to SEQ ID NO:12, wherein amino acids 82-96, 177-191, or 82-96 and 177-191 are retained;
(xiv) a polypeptide comprising SEQ ID NO:9 or SEQ ID NO:
11;
(xv) a polypeptide comprising a polypeptide comprising SEQ ID NO:141, wherein:
(a) W at position 6 is retained and 1, 2, or 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 6 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 7, 8, 9, or 10;
or
(c) Wat position 6 is retained and position 7 is substituted with A, and optionally conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 8, 9, or 10;
(xvi) a polypeptide comprising a polypeptide comprising SEQ ID NO:
151, wherein:
(a) Wat position 6 is retained and 1, 2, or 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 6 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, or 14;
or
(c) Wat position 6 is retained and position 7 is substituted with A, and optionally conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 8, 9, 10, 11, 12, 13, or 14;
(xvii) a polypeptide comprising a polypeptide comprising SEQ ID NO:97, wherein:
(a) W at position 11 is retained and 1, 2, or 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, or 15;
or
(c) Wat position 11 is retained and position 12 is substituted with A, and optionally conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 8, 9, 10, 13, 14, or 15;
(xviii) a polypeptide comprising SEQ ID NO:74, wherein:
(a) K at position 4 and W at position 8 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
or
(b) K at position 4 and W at position 8 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 5, 6, 7, or 10;
(xix) a polypeptide comprising SEQ ID NO:37, wherein:
(a) K at position 4 and W at position 8 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
or
(b) K at position 4 and W at position 8 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 5, 6, 7, 11, 12, 13, 14, or 15;
(xx) a polypeptide comprising SEQ ID NO:54, wherein:
(a) K at position 12, D at position 13, and W at position 14 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) K at position 12, D at position 13, and W at position 14 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 10,
(c) Kat position 12, D at position 13, and Wat position 14 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
7 or 8;
(xxi) a polypeptide comprising SEQ ID NO:140, wherein:
(a) K at position 7, D at position 8, and W at position 9 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) K at position 7, D at position 8, and W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, or 10;
or
(c) K at position 7, D at position 8, and W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
3 or 4.
(xxii) a polypeptide comprising SEQ ID NO:111 wherein:
(a) W at position 10 is retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 10 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, or 15;
or
(c) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 3, 5, 6, 7, 8, 14 or 15.

116 more elements

Dependencies are inferred from the claim text.

Facts

Application: 18/401,086Filed 29 Dec 2023
Priority: 30 Dec 2022
Published: 22 Aug 2024
Term ends: Not estimatedThe source records don’t support an estimate.

Classification12 codes

G01N 33/56911
For microorganisms, e.g. protozoa, bacteria, viruses: bacteria
G01N 33/56933
Bacteria: mycoplasma
C07K 14/195
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from bacteria
C07K 14/30
From bacteria: from Mycoplasmatales, e.g. Pleuropneumonia-like organisms [PPLO]

Family

Application63/477,850Us provisional application · filed 30 Dec 2022
US 2024/0280575 A1This patent

From the US filing history. Foreign family members aren’t listed.

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Citations

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One or more polypeptides comprising one or more of:

(i) a polypeptide as set forth in SEQ ID NO:1, 2, 3, 4, 5, 6, 7, 8, 37, 54, 74, 97, 111, 140, 141, or 151;
(ii) a polypeptide comprising SEQ ID NO:1, wherein:
(a) Wat position 11 is retained and 1, 2, or 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, or 15;
(c) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
2, 3, 4, 6, 7, 8, or 14;
or
(d) W at position 11 is retained and position 12 is substituted with A, and optionally conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 13, 14, or 15;
(iii) a polypeptide comprising SEQ ID NO:2, wherein:
(a) W at position 11 is retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, or 15;
or
(c) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
2, 3, 4, 6, 7, 8, or 14;
(iv) a polypeptide comprising SEQ ID NO:3 wherein:
(a) W at position 10 is retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 10 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, or 15;
or
(c) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 3, 5, 6, 7, 8, 14 or 15;
(v) a polypeptide comprising SEQ ID NO:4, wherein:
(a) W at position 10 is retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 10 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, or 15;
or
(c) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 3, 5, 6, 7, 8, 14 or 15;
(vi) a polypeptide comprising SEQ ID NO:5, wherein:
(a) K at position 9 and Wat position 13 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) K at position 9 and W at position 13 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 14, or 15;
or
(c) K at position 9 and W at position 13 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
2, 3, 4, or 5;
(vii) a polypeptide comprising SEQ ID NO:6, wherein:
(a) K at position 9 and the W at position 13 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) K at position 9 and W at position 13 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 14, or 15;
or
(c) K at position 9 and W at position 13 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
2, 3, 4, or 5;
(viii) a polypeptide comprising SEQ ID NO:7, wherein:
(a) K at position 7, D at position 8, and W at position 9 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) K at position 7, D at position 8, and W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 10, 11, 12, 13, 14, or 15;
or
(c) K at position 7, D at position 8, and W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
3, 4, or 15.
(ix) a polypeptide comprising SEQ ID NO:8, wherein
(a) D at position 8 and W at position 9 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) D at position 8 and W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 10, 11, 12, 13, 14, or 15;
or
(c) D at position 8 and the W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
3, 4, 7, or 15;
(x) a polypeptide comprising SEQ ID NO:10;
(xi) a polypeptide having 95% or more sequence identity to SEQ ID NO:
10, wherein amino acids 75-89, 170-184, or 75-89, and 170-184 are retained;
(xii) a polypeptide comprising SEQ ID NO:
12;
(xiii) a polypeptide having 95% or more sequence identity to SEQ ID NO:12, wherein amino acids 82-96, 177-191, or 82-96 and 177-191 are retained;
(xiv) a polypeptide comprising SEQ ID NO:9 or SEQ ID NO:
11;
(xv) a polypeptide comprising a polypeptide comprising SEQ ID NO:141, wherein:
(a) W at position 6 is retained and 1, 2, or 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 6 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 7, 8, 9, or 10;
or
(c) Wat position 6 is retained and position 7 is substituted with A, and optionally conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 8, 9, or 10;
(xvi) a polypeptide comprising a polypeptide comprising SEQ ID NO:
151, wherein:
(a) Wat position 6 is retained and 1, 2, or 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 6 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, or 14;
or
(c) Wat position 6 is retained and position 7 is substituted with A, and optionally conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 8, 9, 10, 11, 12, 13, or 14;
(xvii) a polypeptide comprising a polypeptide comprising SEQ ID NO:97, wherein:
(a) W at position 11 is retained and 1, 2, or 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, or 15;
or
(c) Wat position 11 is retained and position 12 is substituted with A, and optionally conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 8, 9, 10, 13, 14, or 15;
(xviii) a polypeptide comprising SEQ ID NO:74, wherein:
(a) K at position 4 and W at position 8 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
or
(b) K at position 4 and W at position 8 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 5, 6, 7, or 10;
(xix) a polypeptide comprising SEQ ID NO:37, wherein:
(a) K at position 4 and W at position 8 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
or
(b) K at position 4 and W at position 8 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 5, 6, 7, 11, 12, 13, 14, or 15;
(xx) a polypeptide comprising SEQ ID NO:54, wherein:
(a) K at position 12, D at position 13, and W at position 14 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) K at position 12, D at position 13, and W at position 14 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 10,
(c) Kat position 12, D at position 13, and Wat position 14 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
7 or 8;
(xxi) a polypeptide comprising SEQ ID NO:140, wherein:
(a) K at position 7, D at position 8, and W at position 9 are retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) K at position 7, D at position 8, and W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, or 10;
or
(c) K at position 7, D at position 8, and W at position 9 are retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
3 or 4.
(xxii) a polypeptide comprising SEQ ID NO:111 wherein:
(a) W at position 10 is retained and 1, 2, 3 other amino acids are substituted with a conservative amino acid substitution;
(b) W at position 10 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, or 15;
or
(c) W at position 11 is retained and conservative amino acid substitutions are at 1, 2, or 3 of the following positions:
1, 3, 5, 6, 7, 8, 14 or 15.

116 more elements

Facts

Application

18/401,086Filed 29 Dec 2023

Priority

30 Dec 2022

Published

22 Aug 2024

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

G01N 33/56911
For microorganisms, e.g. protozoa, bacteria, viruses: bacteria
G01N 33/56933
Bacteria: mycoplasma
C07K 14/195
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from bacteria
C07K 14/30
From bacteria: from Mycoplasmatales, e.g. Pleuropneumonia-like organisms [PPLO]