Polypeptide nanopores synthetically functionalized with positively charged species, and methods of making and using the same
AssigneeIllumina, Inc.
InventorsLisa Savagian, Burton Simpson, Sang Park, Boyan Boyanov, Jeffrey G. Mandell, Seth M. McDonald
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Abstract
Polypeptide nanopores synthetically functionalized with positively charged species, and methods of making and using the same, are provided herein. In some examples, a polypeptide nanopore includes a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue. The mutated amino acid residue may be synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel.
Claims27
A polypeptide nanopore comprising a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue which is synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel.
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A method of making a nanopore, the method comprising:
- providing a polypeptide nanopore comprising a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue;
- and
- synthetically functionalizing the mutated amino acid residue with a positively charged species.
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A method of using a nanopore, the method comprising:
- providing a polypeptide nanopore comprising a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue which is synthetically functionalized with a positively charged species;
- inhibiting, using the positively charged species, translocation of cations through the channel;
- and
- translocating anions through the channel.
Dependencies are inferred from the claim text.