Fluorescent probes
AssigneesTohoku Techno Arch Co., Ltd., Goryo Chemical, Inc., Senju Pharmaceutical Co., Ltd.
InventorsToru NAKAZAWA, Yuki SUZUKI, Takayuki OKA, Takatoshi UCHIDA
Life of this patent
Estimating the term…
Abstract
A fluorescent probe that detects calpain enzyme activity with high sensitivity, wherein, enzymatic reactivity with a calpain is improved by bonding an amide group having α-carbon, which is bonded to an oxygen atom, to the N-terminal of a peptide chain. A fluorescent probe detects calpain activity with higher sensitivity because the probe is improved in reactivity with a calpain as compared to a HMRG fluorescent probe that has heretofore been reported. Specifically, the fluorescent probe relates to a compound represented by the following general formula (I) or a salt thereof.
Claims25
A compound represented by the following general formula (I) or a salt thereof:
- where:
- A and B each independently represent an amino acid residue, and the amino acid residues may be identical to or different from each other,
- where a bond between A and NH bonded to A is an amide bond between a C-terminal of A and the NH, A and B are bonded through an amide bond, and a bond between B and a carbonyl group (C═O) bonded to B is an amide bond between an N-terminal of B and the carbonyl group (C═O);
- R1s may be identical to or different from each other, and each represent a substituent bonded to a benzene ring;
- “p” represents an integer of from 0 to 4;
- R2, R3, R4, R5, R6, and R7 each independently represent a hydrogen atom, a hydroxyl group (OH group), a halogen atom, or a linear, branched, or cyclic alkyl group that may be substituted;
- R8 and R9 each independently represent a hydrogen atom, or a linear, branched, or cyclic alkyl group that may be substituted, or R8 and R9 may be bonded to form a ring, or R3 and R8 may be bonded to form a ring, and/or R4 and R9 may be bonded to form a ring;
- X represents a linear or branched C1 to C3 alkylene group;
- R10 and R11 each independently represent a hydrogen atom, a hydroxyl group (OH), an alkoxy group, or a linear, branched, or cyclic alkyl group that may be substituted, or R10 and R11 may be bonded to form a ring;
- R12 represents a hydrogen atom, a linear, branched, or cyclic alkyl group that may be substituted, a linear or branched alkenyl group that may be substituted, a linear or branched alkynyl group that may be substituted, an aryl group that may be substituted, or a heterocyclic group that may be substituted, or R2 may be bonded to R10 or R11 to form a ring;
- and
- L represents an amide bond, an ester bond, or -(linear or branched alkylene-O—)n— where “n” represents an integer of from 1 to 10, or L represents a single bond.
Dependencies are inferred from the claim text.