Pyruvate sensor and related method

Abstract

The present disclosure relates to sensing composition comprising a pyruvate-responsive enzyme and thiamine pyrophosphate (TPP). In particular, the sensing composition can comprise a pyruvate-responsive enzyme (e.g., pyruvate oxidase), TPP, optionally flavin adenine dinucleotide (FAD), a stabilizing agent (e.g., an albumin), and a redox mediator. The sensing composition forms a sensing layer on a working electrode to form a sensing electrode. The sensing electrode, along with a circuit, can form part of a system for sensing pyruvate. The present disclosure is further related to a method for sensing pyruvate, which includes accumulating charge derived from pyruvate reacting with the sensing composition for a set period of time and then measuring a signal from the accumulated charge.

Dependencies are inferred from the claim text.