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US 2024/0279704 A1Published application
Using fucosidase to control afucosylation level of glycosylated proteins
AssigneeAmgen Inc.
InventorsNamratha SUBHASH, Yu Cong CHEN, Kyle Shamus MCELEARNEY, Glen BOLTON
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Abstract
Provided herein are methods of obtaining a recombinant glycosylated protein having increased levels of afucosylated glycoforms. In exemplary embodiments, the methods comprise incubating purified recombinant glycosylated protein with a human broad specificity fucosidase and separating the recombinant glycosylated protein from the fucosidase.
Claims23
A method for obtaining a recombinant glycosylated protein having increased levels of afucosylated glycoforms, the method comprising
- 1) incubating a purified recombinant glycosylated protein with a human broad specificity fucosidase in a buffer suitable for fucosidase activity for a time and under conditions suitable to increase afucosylation of the recombinant glycosylated protein;
- and
- 2) separating the recombinant glycosylated protein having increased levels of afucosylated glycoforms from the fucosidase;
- wherein the recombinant glycosylated protein is not reacted with a glycosyltransferase or sialyltransferase.
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