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AssigneeShenzhen Readline Biotech Co., Ltd.
InventorsTiemei YU, Xiuxiu HE, Guofu QIN, Ruimei LING, Lifeng LIN, Wenjing TAN, Junfeng PAN, Jian LIU
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The present invention relates to the field of biotechnologies. Disclosed are an enzyme composition for preparing β-nicotinamide mononucleotide (NMN), and an application thereof. In the present invention, by using adenosine and nicotinamide as raw materials, D-ribose 1-phosphate and a nicotinamide ribose intermediate are generated under enzyme catalysis of an enzyme composition of a PNP enzyme and an NRK enzyme, and finally NMN is obtained; only two enzymes need to be involved in the whole reaction system, and the by-product adenine can be recycled. Moreover, only one molecule of ATP needs to be consumed for generating one molecule of NMN, thereby greatly reducing the process cost. The reaction of synthesizing NMN by NRK enzyme catalysis in the last step is irreversible, and thus, the substrate conversion rate can be greatly improved and the production cost can be further reduced.
An enzyme composition for preparing β-nicotinamide mononucleotide, consisting of purine nucleoside phosphorylase with an EC number of EC 2.4.2.1 and nicotinamide riboside kinase with an EC number of EC 2.7.1.22;
Dependencies are inferred from the claim text.