Biochip for spatial transcriptomic analysis, manufacturing method therefor and application thereof

A method for analyzing spatial transcriptomic information of a biological tissue sample by using a biochip, said method comprising:

• providing the biochip having an array prepared by steps of
• A. fixing a first set of barcode nucleic acids on the surface of a chip through a plurality of microfluidic channels set in parallel to form a plurality of first barcode strips in a first direction, wherein said first set of barcode nucleic acids comprise a plurality of first barcode nucleic acids having different barcode sequences, each said first barcode strip contains one kind of first barcode nucleic acid, and each said kind of first barcode nucleic acid fixed in each first barcode strip has a different barcode sequence;
• B. applying a second set of barcode nucleic acids in a second direction to said plurality of first barcode strips in the first direction on the chip surface through a plurality of microfluidic channels set in parallel to form a plurality of second barcode strips, said second set of barcode nucleic acids comprise a plurality of second barcode nucleic acids having different barcode sequences, each said second barcode strip contains one kind of second barcode nucleic acid, and each said kind of second barcode nucleic acid contained in each second barcode strip has a different barcode sequence;
• C. under conditions that cause the first barcode nucleic acid and the second barcode nucleic acid to undergo a joining reaction, joining the second barcode nucleic acid to the first barcode nucleic acid on the surface of the chip where said plurality of first barcode strips intersect said plurality of second barcode strips to generate probes, said probes forming arrays of dots, each dot containing a probe with a sequence different from each other;
• and
• contacting the array of the biochip with the tissue sample, the probes on the array recognizing and binding nucleic acids, of cells in the tissue, followed by reverse transcription and/or amplification.