Methods of generating sacral neural crest lineages and uses thereof
AssigneesSloan-Kettering Institute for Cancer Research, Memorial Sloan Kettering Cancer Center, Memorial Hospital for Cancer and Allied Diseases
InventorsLorenz STUDER, Yujie Fan
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Abstract
The present disclosure relates to methods for generating sacral neural crest lineage cells and enteric neurons. Also provided are sacral neural crest lineage cells and enteric neurons generated by the presently disclosed methods and compositions comprising such cells. The present disclosure further provides uses of the sacral neural crest lineage cells and enteric neurons for preventing, modeling, and/or treating of enteric nervous system disorders.
Claims20
An in vitro method for inducing differentiation of stem cells, comprising activation of wingless (Wnt) signaling, activation of fibroblast growth factor (FGF) signaling, and sacral neural crest patterning in the stem cells to obtain a population of differentiated cells expressing at least one marker indicating a sacral neural crest lineage;
- or comprising contacting the stem cells with at least one activator of Wnt signaling, at least one activator of FGF signaling, and at least one molecule that induces sacral neural crest patterning.
A kit for inducing differentiation of stem cells, comprising:
- (a) at least one activator of Wnt signaling;
- (b) at least one activator of FGF signaling;
- (c) at least one molecule that induces sacral neural crest patterning;
- and
- (d) instructions for inducing differentiation of the stem cells into cells expressing at least one sacral neural crest lineage marker;
- or
- comprising:
- (a) at least one activator of Wnt signaling;
- (b) at least one activator of FGF signaling;
- (c) at least one molecule that induces sacral neural crest patterning;
- (d) at least one growth factor,
- (e) at least one Wnt activator;
- and
- (f) instructions for inducing differentiation of the stem cells into cells expressing at least one enteric neuron marker.
Dependencies are inferred from the claim text.