Centrifugal micro-fluidic disk for detecting the presence and concentration of an analyte of interest

A centrifugal micro-fluidic disk comprising:

• an integrated liquid reagent sector including:
• a single-use reagent fluid pack attached to the disk at a liquid reagent input port;
• a reagent fluidic circuit extending radially outward from the liquid reagent input port;
• and
• an reagent distribution ring positioned radially outward from the reagent fluidic circuit;
• and
• an array of sample detection networks, each extending radially outward from a sample input port, wherein:
• in response to an opening of the single-use reagent fluid pack and rotation of the disk, the reagent distribution ring receives reagent moving radially outward from the reagent fluidic circuit and feeds the reagent radially inward into the sample detection networks to fill the detection chambers until the reagent within the sample detection networks achieves a pressure equilibrium with the reagent within the reagent fluidic circuit.

A method of detecting an analyte of interest within a fluid sample comprising:

• combining the fluid sample including the analyte of interest and one or more metabolites of the analyte of interest with a first additive including magnetic beads within a first chamber, each of the magnetic beads including an antibody attached thereto, wherein the antibody is specific to the metabolites;
• forming magnetic bead combinations of the magnetic beads and the metabolites;
• outputting the combined fluid sample and first additive from the first chamber to a second chamber;
• attracting the magnetic beads and the magnetic bead combinations to a magnet applied to an exterior of a second chamber;
• and
• outputting a magnetically pre-separated sample of the fluid sample and the first additive from the second chamber.