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US 2024/0269286 A1Published application
Methods and compositions for decreasing chronic pain
AssigneeEOS Neuroscience, Inc.
InventorsAlan Horsager, Kenneth Greenberg, Benjamin C. Matteo, Edward S. Boyden, Douglas G. Ririe, James C. Eisenach, Christian Wentz
Life of this patent
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Abstract
Provided are compositions and methods for the selective silencing of neurons in pain pathway by using a combination of inhibitory light-sensitive protein gene transfer and wavelength specific illumination.
Claims21
1-35. (canceled)
A method to relieve neuropathic pain in a subject in need of such relief, comprising
- transfecting dorsal root ganglion (DRG) neurons of the subject with an adenoaassociated viral (AAV) vector comprising a polynucleotide, wherein the polynucleotide comprises a regulatory region driving expression of a sequence encoding an opsin, wherein
- the regulatory region comprises one or more of a preprotachykinin-A (PPT) promoter, a voltage-gated sodium channel subunit alpha (Scn10a) promoter, and a vanilloid receptor subtype 1 (TRPV1) promoter;
- and
- exposing DRG neurons expressing the opsin to a wavelength of light wherein exposure to the wavelength causes an increase in rheobase for the DRG neurons expressing the opsin.
1 more element
A method to relieve neuropathic pain in a subject in need of such relief, comprising
- transfecting dorsal root ganglion (DRG) neurons of the subject with an adenoaassociated viral (AAV) vector comprising a polynucleotide, wherein the polynucleotide comprises a regulatory region driving expression of a sequence encoding an opsin, wherein
- the regulatory region comprises one or more of a preprotachykinin-A (PPT) promoter, a voltage-gated sodium channel subunit alpha (Scn10a) promoter, and a vanilloid receptor subtype 1 (TRPV1) promoter;
- and
- the opsin is selected from rhodopsin, blue opsin, red opsin, halorhodopsin, channelrhodopsin-2, enhanced halorhodopsin, archaerhodopsin-3, and leptosphaeria maculans, and
- exposing DRG neurons expressing the opsin to a wavelength of light wherein exposure to the wavelength causes an increase in rheobase for the DRG neurons expressing the opsin.
2 more elements
A method to relieve neuropathic pain in a subject in need of such relief, comprising
- transfecting dorsal root ganglion (DRG) neurons of the subject with an adenoaassociated viral (AAV) vector comprising a polynucleotide, wherein the polynucleotide comprises a regulatory region driving expression of a sequence encoding an opsin, wherein
- the regulatory region comprises one or more of a preprotachykinin-A (PPT) promoter, a voltage-gated sodium channel subunit alpha (Scn10a) promoter, and a vanilloid receptor subtype 1 (TRPV1) promoter;
- and
- the opsin is selected from halorhodopsin, enhanced halorhopopsin, archaerhodopsin-3, and leptosphaeria maculans, and
- exposing DRG neurons expressing the opsin to a wavelength of light wherein exposure to the wavelength causes an increase in rheobase for the DRG neurons expressing the opsin.
2 more elements
Dependencies are inferred from the claim text.