Method of preparing a whey-derived composition enriched in phospholipids and osteopontin, the composition as such, and nutritional use of the composition
OwnerHans BERTELSEN, Nikolaj DRACHMANN +3inventors
InventorsHans BERTELSEN, Nikolaj DRACHMANN, Anne Staudt KVISTGAARD, Kristian Raaby POULSEN, Donata VOSYLE
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Abstract
The invention relates to a method of preparing a whey-derived composition enriched in whey phospholipids and osteopontin (OPN) by membrane filtration, and preferably also enriched in other membrane components from whey. The invention furthermore relates to the whey-derived composition as such, use of the whey-derived composition for increasing the content of OPN in nutritional products, and to nutritional products comprising the whey-derived composition.
Claims39
A method of preparing a whey-derived composition enriched with respect to phospholipid and osteopontin (OPN), and preferably also enriched with respect to other milk fat globule membrane components, the method comprising the steps of:
- a) providing a liquid feed comprising whey protein including osteopontin and alpha-lactalbumin (ALA) and phospholipid originating from whey, the liquid feed containing a total amount of osteopontin in the range of 0.2-2.0% w/w relative to total protein, the liquid feed preferably having a pH in the range of 4.0-8, more preferably 5.5-7.5, even more preferably 5.7-7.0, and most preferably 5.9-6.6.
- b) subjecting the liquid feed to membrane filtration to provide a filtration retentate and a filtration permeate, said membrane filtration is arranged and operated to:
- provide a content of osteopontin on total protein basis of the filtration retentate that is at least 150% of the content of osteopontin on total protein basis of the liquid feed, and
- preferably, to provide a content of total phospholipid relative to total solids of the filtration retentate that is at least 200% relative to total solids of the liquid feed, and
- provide a content of alpha-lactalbumin on total protein basis of the filtration retentate that is at most 75% of the content of alpha-lactalbumin on total protein basis of the liquid feed, and
- optionally c) subjecting the filtration retentate or a product stream comprising at least lipid and protein originating from the filtration retentate to one or more additional processing steps, preferably comprising one or more of:
- i) microfiltration,
- ii) concentration,
- iii) heat-treatment, and
- iv) drying.
The method according to any of the preceding claims wherein the whey of step a) is a sweet whey or an acid whey.
The method according to any of the preceding claims wherein the liquid feed comprises total protein in an amount in the range of 5-89% w/w relative to total solids, more preferably 30-86% w/w, even more preferably 40-83% w/w, and most preferably 60-80% w/w relative to total solids.
The method according to any of the preceding claims wherein the liquid feed comprises a total amount of alpha-lactalbumin in the range of 5-40% w/w relative to total protein, more preferably 10-35% w/w, even more preferably 10-30% w/w, and most preferably 10-25% w/w relative to total protein.
The method according to any of the preceding claims wherein the liquid feed comprises a total amount of osteopontin in the range of 0.2-0.9% w/w relative to total protein, more preferably 0.3-0.8% w/w, even more preferably 0.4-0.8% w/w, and most preferably 0.4-0.7% w/w relative to total protein or wherein the liquid feed comprises a total amount of osteopontin in the range of 1.0-2.0% w/w relative to total protein, more preferably 1.2-2.0% w/w, even more preferably 1.3-2.0% w/w, and most preferably 1.4-2.0% w/w relative to total protein.
The method according to any of the preceding claims wherein the liquid feed comprises a total amount of phospholipid in the range of 0.2-5% w/w relative to total solids, more preferably 0.4-4% w/w, even more preferably 0.5-3% w/w, and most preferably 1-3% w/w relative to total solids.
The method according to any of the preceding claims wherein the liquid feed comprises a total amount of phospholipid derived from milk extracellular vesicles (milk EV) in an amount of at least 50% w/w relative to total phospholipid, more preferably at least 54% w/w, even more preferably at least 56% w/w, and most preferably at least 58% w/w.
The method according to any of the preceding claims wherein the liquid feed comprises a total amount phospholipid derived from milk EV in an amount of 50-75% w/w relative to total phospholipid, more preferably 54-73% w/w, even more preferably 56-71% w/w, and most preferably 58-70% w/w.
The method according to any of the preceding claims wherein the liquid feed comprises a total amount phospholipid derived from milk EV in an amount of at least 76% w/w relative to total phospholipid, more preferably at least 80% w/w, even more preferably at least 85% w/w, and most preferably at least 90% w/w.
The method according to any of the preceding claims wherein the liquid feed comprises total protein in an amount of 0.2-8% w/w relative to the weight of the liquid feed, more preferably 1-7% w/w, even more preferably 2-6% w/w, and most preferably 2-5% w/w relative to the weight of the liquid feed.
The method according to any of the preceding claims wherein the membrane filtration of step b) is arranged and operated to provide a content of alpha-lactalbumin on total protein basis of the filtration retentate that is at most 50% of the content of alpha-lactalbumin on total protein basis of the liquid feed, more preferably at most 30%, even more preferred at most 20% and most preferred at most 10% of the content of alpha-lactalbumin on total protein basis of the liquid feed.
The method according to any of the preceding claims wherein the membrane filtration of step b) is arranged and operated to provide a content of osteopontin on total protein basis of the filtration retentate that is at least 180% of the content of osteopontin on total protein basis of the liquid feed, more preferably at least 200%, even more preferably at least 230%, and most preferably at least 250% of the content of osteopontin on total protein basis of the liquid feed.
The method according to any of the preceding claims wherein the membrane filtration of step b) is arranged and operated to provide a content of total phospholipid relative to total solids of the filtration retentate that is at least 225%, even more preferably at least 250%, and most preferably at least 275% of the content of total phospholipid relative to total solids of the liquid feed.
The method according to any of the preceding claims wherein the membrane filtration of step b) involves one or more membrane(s) with a nominal molecular weight cut-off in the range of 100-2000 kDa, more preferably 300-1600 kDa;
- even more preferably 500-1300 kDa, and most preferably 700-1000 kDa.
The method according to any of the preceding claims wherein the membrane filtration of step b) involves diafiltration.
The method according to any of the preceding claims wherein the membrane filtration of step b) is operated with
- with a trans-membrane pressure of 0.1-5 bar, more preferably 0.2-3 bar and most preferably 0.3-1 bar, and/or
- at a temperature of 1-60 degrees C., more preferably 2-30 degrees C., even more preferably 5-20 degrees C., and most preferably 8-15 degrees C.
A whey-derived composition comprising:
- total lipid in the amount of 10 to 30% w/w relative to total solids,
- total phospholipid in an amount of 3 to 12% w/w relative to total solids,
- an ash content in the range of 1-10% w/w relative to total solids,
- lactose in the amount of at most 10% w/w relative to total solids;
- total protein in an amount of 65 to 80% w/w relative to total solids,
- OPN in an amount of 0.8-5% w/w relative to total protein.
Dependencies are inferred from the claim text.