System and method for carrying out morphometric analysis of the vascularization of an organ

A method for generating a morphometric quantity of interest of a section of a human or animal organ, from a first digital representation of a histological section, said first digital representation consisting of a pixel table each pixel encoding a set of integer values respectively describing luminous intensities of primary colours, said method being implemented by a processing unit of an electronic object, said electronic object also including an output human-machine interface and a data memory, characterized in that:

• a. the histological section was subjected to a staining step, prior to its digitization, in order to generate said first digital representation, said staining causing distinct colourings of the pixels of said first digital representation when these latter depict a blank, tissue and muscle cells;
• and
• b. said method includes:
• i. a step of distinguishing the pixels of said first digital representation depicting tissue from those depicting a blank and forming a “parenchyma mask” in the form of a second digital representation having the same dimensions as the first digital representation, each pixel of which depicts:
• a first characteristic value when the corresponding pixel in the first digital representation depicts tissue;
• a second characteristic value when said corresponding pixel in the first digital representation depicts a blank;
• ii. a step of distinguishing the pixels of said first digital representation depicting muscle cells and forming a “muscle cells mask” in the form of a third digital representation having the same dimensions as the first digital representation, each pixel of which depicts:
• a first characteristic value when the corresponding pixel in the first digital representation depicts a muscle cell;
• a second characteristic value otherwise;
• iii. an iterative step of analysis of the second and third digital representations consisting of:
• identifying the pixels depicting at least one polygon of interest within one of the second and third digital representations;
• distinguishing, from said second and third digital representations and from the at least one identified polygon of interest, a lumen, an intima and a media of a vessel;
• generating at least one morphometric measurement from said lumen, intima and/or media of said vessel and storing said at least one morphometric measurement in the data memory;
• iv. a step of generating a morphometric quantity of interest of the tissue of the human or animal organ from at least one morphometric measurement of a vessel stored in the data memory;
• and
• v. a step of causing an output of said morphometric quantity of interest via the output human-machine interface.