Methods of monitoring in vitro transcription of mrna and/or post-in vitro transcription processes
AssigneeModernaTX, Inc.
InventorElliott Schmitt
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Abstract
Methods of monitoring in vitro transcription of mRNA and/or post-in vitro transcription processes are provided. For example, methods of monitoring in vitro transcription of mRNA and/or post-in vitro transcription processes by acquiring one or more Raman spectra are provided.
Claims32
A method, wherein the method comprises acquiring one or more Raman spectra during in vitro transcription of mRNA.
The method of any preceding claim, wherein the method further comprises monitoring one or more reaction conditions and/or progression of the in vitro transcription.
The method of any preceding claim, wherein the method further comprises determining whether the in vitro transcription has reached a desired endpoint, whether the in vitro transcription is progressing at a desired rate, and/or whether one or more reaction conditions are as desired.
The method of any preceding claim, wherein the method comprises using greater than or equal to 1 probe for Raman spectroscopy and less than or equal to 10 probes for Raman spectroscopy.
The method of any preceding claim, wherein the in vitro mRNA transcription comprises a batch process, a fed-batch process, and/or a continuous process.
(canceled)
Dependencies are inferred from the claim text.