US 2024/0263204 A1 · METHOD FOR PREPARING S-NICOTINE

Abstract

The present invention relates to the technical field of biosynthesis, and in particular to a method for preparing S-nicotine. Amine oxidase is utilized to oxidize 1-methylpyrrolidine into corresponding imine, and then the imine and nicotinic acid are condensed and decarboxylated under the catalysis of nicotine synthetase to obtain a final product S-nicotine. The S-nicotine having specific chirality can be obtained by means of two-step reaction in a reaction system, the synthetic route is short, the yield is high, the reaction conditions are mild, and large-scale production is easy to achieve; moreover, raw materials are wide in source, low in price, low in production cost and environmentally friendly, the production cost of nicotine is remarkably reduced, and the requirements of current green industrial production can be better satisfied.

Claims21

Dependent claims

An amine oxidase mutant, wherein an amino acid sequence of the amine oxidase mutant is selected from the group consisting of:

amino acid sequence set forth in SEQ ID NO:
1 or SEQ ID NO:
2,
amino acid sequence derived from SEQ ID NO:
1 or SEQ ID NO:
2 by substitution, deletion or addition of one or more amino acids and functionally identical or similar to SEQ ID NO:
1 and SEQ ID NO:
2, and
amino acid sequence having at least 90% homology to the amino acid sequence set forth in SEQ ID NO:
1 or SEQ ID NO:
2 and functionally identical or similar to amino acid sequence set forth in SEQ ID NO:
1 or SEQ ID NO:
2.

10 more elements

Dependencies are inferred from the claim text.

Method for preparing s-nicotine

Life of this patent

Method for preparing s-nicotine

Life of this patent

Abstract

Claims21