Production of saturated fats in microbes
AssigneeNourish Ingredients Pty Ltd
InventorsAnna EL TAHCHY, Dawar HUSSEIN, Bei DONG, Geoffrey John DUMSDAY, Robert Charles DE FEYTER, Surinder Pal SINGH, James Robertson PETRIE
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Abstract
The present invention relates to extracted microbial lipids, microbial cells comprising the lipid, and extracts thereof. The present invention also relates to use of these lipids, cells and extracts in foods, feedstuffs and beverages.
Claims71
Extracted microbial lipid comprising a total fatty acid content which comprises a total saturated fatty acid content of saturated fatty acids (SFA) and a total monounsaturated fatty acid content of monounsaturated fatty acids (MUFA), wherein at least some of the total fatty acid content including at least some of the SFA and at least some of the MUFA is esterified in the form of triacylglycerols (TAG) such that the extracted microbial lipid has a total TAG content, and wherein
- (i) the total SFA content of the extracted microbial lipid comprises stearic acid (C18:0), palmitic acid (C16:0), myristic acid (C14:0), arachidic acid (C20:0), behenic acid (C22:0) and lignoceric acid (C24:0), whereby at least 50% by weight of the fatty acids in the total fatty acid content of the extracted microbial lipid are SFA;
- (ii) between 20% and 85% by weight of the total fatty acid content is stearic acid;
- (iii) the total MUFA content of the extracted microbial lipid comprises oleic acid (C18:1Δ9) and palmitoleic acid (C16:1Δ9), and optionally C16:1Δ7 and/or C17:1;
- (iv) the total fatty acid (TFA) content of extracted microbial lipid either lacks polyunsaturated fatty acids (PUFA) or comprises a PUFA content which comprises linoleic acid (C18:2Δ9,12), wherein the PUFA content is less than 5% by weight of the total fatty acid content;
- (v) the extracted microbial lipid either comprises polar lipid comprising phospholipid, or lacks polar lipid,
- (vi) the extracted microbial lipid is a solid at 25° C., and
- (vii) the extracted microbial lipid was obtained from microbial cells, wherein the microbial cells are yeast cells.
Extracted microbial lipid comprising a total fatty acid content which comprises a total saturated fatty acid content of saturated fatty acids (SFA) and a total monounsaturated fatty acid content of monounsaturated fatty acids (MUFA), wherein at least some of the total fatty acid content including at least some of the SFA and at least some of the MUFA is esterified in the form of triacylglycerols (TAG) such that the extracted microbial lipid has a total TAG content, and wherein
- (i) the total SFA content of the extracted microbial lipid comprises stearic acid (C18:0), palmitic acid (C16:0), myristic acid (C14:0), arachidic acid (C20:0), behenic acid (C22:0) and lignoceric acid (C24:0), whereby at least 50% by weight of the fatty acids in the total fatty acid content of the extracted microbial lipid are SFA;
- (ii) between 20% and 85% by weight of the total fatty acid content is stearic acid;
- (iii) the total MUFA content of the extracted microbial lipid comprises oleic acid (C18:1Δ9) and palmitoleic acid (C16:1Δ9), and optionally C16:1Δ7 and/or C17:1;
- (iv) the total fatty acid (TFA) content of extracted microbial lipid either lacks polyunsaturated fatty acids (PUFA) or comprises a PUFA content which comprises linoleic acid (C18:2Δ9,12), wherein the PUFA content is less than 5% by weight of the total fatty acid content;
- (v) the extracted microbial lipid either comprises polar lipid comprising phospholipid, or lacks polar lipid, and
- (vi) the extracted microbial lipid is a solid at 25° C.
Microbial cells having at least one genetic modification, wherein the microbial cells have (i) an increased saturated fatty acid (SFA) content, an increased content of SFA having at least 18 carbons, an increased content of stearate, an increased content of C20:0 and C22:0 fatty acids, an increased content of C24:0 fatty acid, an increased L/S-SFA ratio, or any combination thereof, in the total fatty acid (TFA) content of the microbial cells or the TFA content of TAG in the microbial cells, or both, and (ii) increased triacylglycerol (TAG) production or accumulation, or both, in each case when compared to corresponding microbial cells lacking the at least one genetic modification and cultured under the same conditions.
Dependencies are inferred from the claim text.