Methods and microorganisms for making 2,3-butanediol and derivatives thereof from c1 carbons
AssigneePrecigen, Inc.
InventorsXinhua ZHAO, Mark Anton HELD, Tina HUYNH, Lily Yuin CHAO, Na TRINH, Matthias Helmut SCHMALISCH, Bryan YEH, James KEALEY, Kevin Lee DIETZEL
Life of this patent
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Abstract
Genetically modified microorganisms that have the ability to convert carbon substrates into chemical products such as 2,3-BDO are disclosed. For example, genetically modified methanotrophs that are capable of generating 2,3-BDO at high titers from a methane source are disclosed. Methods of making these genetically modified microorganisms and methods of using them are also disclosed.
Claims35
(canceled)
The microorganism of claim 128, comprising:
- (a) a heterologous acetoin reductase;
- (b) a heterologous alpha-acetolactate decarboxylase;
- and/or (c) a heterologous acetolactate synthase.
3-5. (canceled)
(canceled)
10-13. (canceled)
The microorganism of claim 128, wherein the microorganism is a methanotroph.
16-16. (canceled)
The microorganism of claim 128, wherein the microorganism is Methylococcus capsulatus.
18-31. (canceled)
A vector comprising two or more genes encoding an acetoin reductase, an alpha-acetolactate decarboxylase, and/or an acetolactate synthase.
33-41. (canceled)
A method of making the microorganism of claim 128, the method comprising transforming a microorganism with a heterologous nucleic acid encoding an acetoin reductase, an alpha-acetolactate decarboxylase, and/or an acetolactate synthase.
43-62. (canceled)
64-69. (canceled)
72-74. (canceled)
78-88. (canceled)
90-127. (canceled)
A genetically modified microorganism capable of converting a C1 carbon to 2,3-BDO, the microorganism comprising at least one heterologous gene encoding;
- (a) an acetoin reductase;
- (b) an alpha-acetolactate decarboxylase;
- and/or (c) an acetolactate synthase.
(canceled)
133-134. (canceled)
136-146. (canceled)
Dependencies are inferred from the claim text.