Cell cultivation method, cell support composite production method, cultivated cells, and cell support composite
AssigneeNikkiso Co., Ltd.
InventorFumihiko KITAGAWA
Life of this patent
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Abstract
Cultivated cells that are produced by a cultivation method including cultivating primary cultured kidney cells which are dedifferentiated in a state of being non-adherent to a culture vessel for a period of 5 days or longer, forming aggregates of the kidney cells during the cultivation period, then cultivating the kidney cells in a state of having formed aggregates, during a portion of the period, and thereby restoring physiological functions of the kidney cells. The primary cultured kidney cells include primary cultured renal proximal tubular epithelial cells, and a percentage of the primary cultured renal proximal tubular epithelial cells in the primary cultured kidney cells being 83% or more.
Claims9
Cultivated cells being produced by a cultivation method including:
- cultivating primary cultured kidney cells which are dedifferentiated in a state of being non-adherent to a culture vessel for a period of 5 days or longer, forming aggregates of the kidney cells during the cultivation period, then cultivating the kidney cells in a state of having formed aggregates, during a portion of the period, and thereby restoring physiological functions of the kidney cells, the primary cultured kidney cells including primary cultured renal proximal tubular epithelial cells, and a percentage of the primary cultured renal proximal tubular epithelial cells in the primary cultured kidney cells being 83% or more.
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