Cell cultivation method, cell support composite production method, cultivated cells, and cell support composite

A method for producing a cell support composite, the method comprising:

• applying a coating agent containing one or more selected from the group consisting of laminin molecules, a basement membrane matrix mixture, collagen molecules, and fragments of any of these, on at least a portion of a substrate;
• separating aggregates formed by a cell cultivation method into individual cultivated cells, the cultivation method including cultivating primary cultured kidney cells which are dedifferentiated in a state of being non-adherent to a culture vessel for a period of 5 days or longer, forming aggregates of the kidney cells during the cultivation period, then cultivating the kidney cells in a state of having formed aggregates during a portion of the period, and thereby restoring physiological functions of the kidney cells, the primary cultured kidney cells including primary cultured renal proximal tubular epithelial cells, and a percentage of the primary cultured renal proximal tubular epithelial cells in the primary cultured kidney cells being 83% or more;
• and
• seeding the cultivated cells onto the substrate coated with the coating agent, cultivating the cultivated cells on the substrate, and thereby forming a single layer structure of the cultivated cells.