Glycosylated comp pilin variants, methods of making and uses thereof
AssigneeVaxnewmo LLC
InventorsChristian HARDING, Mario FELDMAN
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Abstract
Provided herein are glycosylated ComP proteins, fragments and fusion proteins thereof, and methods of making, for example, for use in the production of conjugate vaccines. Also provided herein are conjugate vaccines against diseases including bacterial diseases.
Claims80
A bioconjugate comprising an oligo- or polysaccharide covalently linked to a fusion protein, wherein the fusion protein comprises a ComP protein (ComP) or a glycosylation tag fragment thereof.
A ComP glycosylation tag comprising an isolated fragment of a ComP protein, wherein the fragment comprises a serine residue corresponding to the conserved serine residue at position 84 in SEQ ID NO:
- 1 (ComPADP1:
- AAC45886.1).
A method of in vivo conjugation of an oligo- or polysaccharide to an acceptor polypeptide, the method comprising covalently linking the oligo- or polysaccharide to the acceptor polypeptide with a PglS oligosaccharyltransferase (OTase), wherein the acceptor polypeptide comprises a ComP protein or a glycosylation tag fragment thereof;
- optionally
- wherein the ComP protein or glycosylation tag fragment thereof is linked to a heterologous carrier protein.
A host cell comprising (a) a genetic cluster encoding for the proteins required to synthesize an oligo- or polysaccharide;
- (b) a PglS OTase;
- and (3) an acceptor polypeptide comprising a ComP protein or a glycosylation tag fragment thereof.
Dependencies are inferred from the claim text.