Method and reagents for the detection of an autoantibody
AssigneesFundació De Recerca ClíNic Barcelona-Institut D'Investigacions BiomèDiques August Pi I Sunyer, Institució Catalana De Recerca I Estudis AvançAts (Icrea)
InventorsJosep Dalmau, Lidia Sabater, Francesc Graus
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Abstract
A method involves detecting in a sample an autoantibody binding specifically to one or more from Gluk1, Gluk2, Gluk3, Gluk4 and Gluk5, preferably Gluk2. An autoantibody binds specifically to one or more from Gluk1, Gluk2, Gluk3, Gluk4 and Gluk5, preferably Gluk2. A diagnostically useful carrier with a solid phase with an immobilized polypeptide contains one or more from Gluk1, Gluk2, Gluk3, Gluk4 and Gluk5, preferably Gluk2 or a variant thereof. The autoantibody can be used for diagnosing a neurological autoimmune disease or a cancer.
Claims20
A method, comprising:
- detecting in a sample an autoantibody binding specifically to one or more selected from the group consisting of Gluk1, Gluk2, Gluk3, Gluk4, and Gluk5.
A device, comprising:
- a carrier with a solid phase with one or more polypeptides each of which comprising one or more antigens selected from the group consisting of Gluk1, Gluk2, Gluk3, Gluk4, Gluk5, and a variant thereof, immobilized on the solid phase, and a) a negative control and/or b) at least one additional antigen, wherein the one or more polypeptides and the negative control or the at least one additional antigen are spatially separated on the carrier,
- or
- a first carrier with a solid phase comprising the one or more polypeptides, immobilized on the solid phase, and a second carrier comprising a solid phase with an immobilized a) negative control and/or b) at least one additional immobilized polypeptide comprising an antigen or a variant thereof.
A kit, comprising:
- a polypeptide comprising one or more selected from the group consisting of Gluk1, Gluk2, Gluk3, Gluk4, Gluk5, and a variant thereof, and a carrier, wherein
- a) the polypeptide is immobilized on the carrier, and the kit further comprises a means for detecting an autoantibody binding specifically to one or more selected from the group consisting of Gluk1, Gluk2, Gluk3, Gluk4, and Gluk5,
- b) the polypeptide and the carrier are configured for immobilizing the polypeptide on a surface of the carrier, and the kit further comprises a means for detecting an immobilized antibody binding specifically to one or more selected from the group consisting of Gluk1, Gluk2, Gluk3, Gluk4, and Gluk5,
- c) the carrier is immobilized with a means for capturing an antibody and the kit further comprises a means for detecting an immobilized antibody binding specifically to one or more selected from the group consisting of Gluk1, Gluk2, Gluk3, Gluk4, and Gluk5,
- d) the carrier and a means for capturing an antibody are configured for immobilizing the means for capturing an antibody on a surface of the carrier, and the kit further comprises a means for detecting an immobilized antibody binding specifically to one or more selected from the group consisting of Gluk1, Gluk2, Gluk3, Gluk4, and Gluk5, or
- e) the polypeptide is present in the form of a cell overexpressing the polypeptide, wherein the cell is the carrier.
Dependencies are inferred from the claim text.