Back to results
US 2024/0254658 A1Published application
Methods and systems for analysis
Assignee10x Genomics, Inc.
InventorsJason Bell, Francesca Meschi, Geoffrey McDermott, Michael Schnall-Levin, Xinying Zheng
Life of this patent
Estimating the term…
Abstract
Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.
Claims30
A method of analyzing chromatin, comprising:
- (a) providing a mixture comprising (i) a biological particle comprising (1) chromatin comprising a template deoxyribonucleic acid (DNA) and (2) a protein, and (ii) a plurality of nucleic acid barcode molecules;
- (b) contacting said biological particle with a labelling agent comprising a reporter oligonucleotide, wherein said labelling agent is configured to couple to said protein;
- (c) generating a plurality of template DNA fragments of said chromatin using a plurality of transposase complexes;
- (d) generating a first barcoded nucleic acid molecule using (i) a template DNA fragment of said plurality of template DNA fragments and (ii) a first nucleic acid barcode molecule of said plurality of nucleic acid barcode molecules;
- and
- (e) generating a second barcoded nucleic acid molecule using (i) said reporter oligonucleotide and (ii) a second nucleic acid barcode molecule of said plurality of nucleic acid barcode molecules.
3 more elements
Dependencies are inferred from the claim text.