Diamond magnetometry detection of biological targets
AssigneeColumbus Nanoworks, Inc.
InventorsArfaan RAMPERSAUD, Isaac RAMPERSAUD, Charles FLECTCHER, Mia FORREN
Life of this patent
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Abstract
A composition includes a fluorescent nitrogen-vacancy nanodiamond conjugated to a probe. The probe specifically binds to a target material when present in a biological sample. A device includes a flow cell containing a plurality of fluorescent nitrogen-vacancy nanodiamonds immobilized on a surface of the flow cell. Each of the plurality of fluorescent nitrogen-vacancy nanodiamonds is conjugated to a probe. A diamond magnetometry system includes an ODMR measurement system, a spin-lattice relaxation time (T1) system, a spin-spin relaxation time (T2) measurement system, and a flow cell.
Claims20
A system for detecting a target material, the system comprising:
- a plurality of fluorescent nitrogen-vacancy nanodiamond-probe-reporter conjugates (MND-probe conjugates), each MND-probe conjugate comprising:
- at least one fluorescent nitrogen-vacancy nanodiamond (FND);
- at least one reporter selected from the group consisting of a magnetic particle (MP), a paramagnetic complex, and a chemical;
- and
- at least one polymeric probe comprising a biomolecule selected from the group consisting of at least one polynucleotide, at least one polypeptide, and a combination thereof, wherein the polymeric probe is characterized as having binding specificity to the target material;
- wherein each of the at least one FND and the at least one reporter is linked to the at least one polymeric probe, and
- wherein a distance between the FND and the reporter in the MND-probe conjugate changes when the at least one polymeric probe binds to the target material and displaces or releases the target material.
A reagent for detection of a target material, the reagent comprising:
- a plurality of fluorescent nitrogen-vacancy nanodiamond-probe-reporter conjugates (MND-probe conjugates), each MND-probe conjugate comprising:
- at least one fluorescent nitrogen-vacancy nanodiamond (FND);
- at least one reporter comprising a magnetic nanoparticle particle (MNP);
- and
- at least one polymeric probe, the polymeric probe comprising a first nucleic acid strand coupled to the MP and a second nucleic acid strand coupled to the FND, the first nucleic acid strand being at least partially complementary to the second nucleic acid strand to releasably couple the MP to the FND via the interaction between the complementary first and second nucleic acid strands, one of the nucleic acid strands having binding affinity for the target material;
- wherein a distance between the FND and the reporter in the MND-probe conjugate changes when the at least one polymeric probe binds to the target material and displaces or releases the target material.
Dependencies are inferred from the claim text.