Proximity oligonucleotides and methods of use thereof

A method of generating an amplification product, said method comprising:

• a) contacting a target polynucleotide in a cell or tissue with a first oligonucleotide comprising a first target hybridization sequence, a first hybridization sequence, a barcode sequence, and a second hybridization sequence, and hybridizing the first target hybridization sequence to a first target sequence of said target polynucleotide;
• b) contacting the target polynucleotide with a second oligonucleotide comprising a second target hybridization sequence and a third hybridization sequence, and hybridizing the second target hybridization sequence to a second target sequence of said target polynucleotide;
• c) hybridizing a circularizable oligonucleotide to both said first oligonucleotide and said second oligonucleotide, wherein said circularizable oligonucleotide comprises a first sequence complementary to said first hybridization sequence, a second sequence complementary to said second hybridization sequence, and a third sequence complementary to said third hybridization sequence;
• d) extending said circularizable oligonucleotide along the barcode sequence with a polymerase to generate a complementary barcode sequence and ligating the complementary barcode sequence to said circularizable oligonucleotide, thereby generating a circular oligonucleotide;
• and
• e) amplifying the circular oligonucleotide, thereby generating an amplification product comprising multiple complements of the complementary barcode sequence.