Selective oxidation of 5-methylcytosine by tet-family proteins
AssigneesChildren's Medical Center Corporation, The United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsAnjana Rao, Mamta Tahiliani, Kian Peng Koh, Suneet Agarwal, Aravind Iyer
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Abstract
The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.
Claims31
1-30. (canceled)
A method for detecting a methylated cytosine residue in a nucleic acid, comprising:
- (a) contacting said nucleic acid with a polypeptide having at least 90% sequence identity with a TET1 polypeptide, a TET2 polypeptide, a TET3 polypeptide, a CXXC4 polypeptide, a catalytically active fragment thereof, a variant thereof, or any combination thereof to generate a product nucleic acid;
- and
- (b) performing a reaction on said product nucleic acid or a derivative thereof, to identify a presence of said methylated cytosine residue in said nucleic acid.
Dependencies are inferred from the claim text.