Systems and methods for cell-free polypeptide synthesis
AssigneeNational Resilience, Inc.
InventorsDanielle Jessica Yoesep, Weston Kevin Kightlinger, Sajita Caroni Shah-Morales
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Abstract
Disclosed herein is a method for cell-free polypeptide synthesis. The method comprises lysing a cell to produce a cellular lysate, passing the cellular lysate through a filter to produce a cellular extract, contacting the cellular extract with a nucleic acid encoding a polypeptide to produce a reaction mixture, and incubating the reaction mixture to produce the polypeptide.
Claims36
A method for cell-free polypeptide synthesis comprising:
- (a) lysing a cell to produce a cellular lysate;
- (b) passing the cellular lysate through a filter to produce a cellular extract;
- (c) contacting the cellular extract with a nucleic acid encoding a polypeptide to produce a reaction mixture;
- and
- (d) incubating the reaction mixture to produce the polypeptide.
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Dependencies are inferred from the claim text.