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AssigneeMonsanto Technology LLC
InventorsBrent BROWER-TOLAND, Shunhong DAI, Karen GABBERT, Alexander GOLDSHMIDT, Miya HOWELL, Brad MCDILL, Dan OVADYA, Beth SAVIDGE, Vijay SHARMA
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The present invention provides recombinant DNA constructs, vectors and molecules useful for attenuating and/or refining the expression of a florigenic FT gene or transgene using targeting sequences of small RNA molecules. Transgenic plants, plant cells and tissues, and plant parts comprising the recombinant constructs, vectors, and molecules are also provided. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant via suppression, relative to a control or wild type plant. Methods are further provided for introducing the recombinant DNA constructs, vectors, and molecules into a plant, and planting transgenic plants in the field including at higher densities. Transgenic plants of the present invention may provide greater yield potential than wild type or control plants.
A recombinant DNA construct comprising a first expression cassette and a second expression cassette, wherein the first expression cassette comprises a polynucleotide sequence encoding a florigenic FT protein operably linked to a first plant expressible promoter, and the second expression cassette comprises a transcribable DNA sequence encoding a RNA molecule comprising a targeting sequence that is at least 80% complementary to at least 15 consecutive nucleotides of the polynucleotide sequence of the first expression cassette, and wherein the transcribable DNA sequence is operably linked to a second plant expressible promoter.
A recombinant DNA construct comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a plant expressible promoter, wherein the polynucleotide sequence comprises a sequence that encodes a target site in a mRNA transcript encoded by the polynucleotide sequence, and wherein the target site of the mRNA transcript is at least 80% complementary to an endogenous RNA molecule.
A transgenic plant comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, wherein expression of the florigenic FT protein is suppressed in a late vegetative and/or reproductive tissue.
A recombinant DNA construct comprising a polynucleotide sequence encoding a florigenic FT protein and operably linked to a vegetative stage promoter, and at least one transcribable DNA sequence encoding an RNA molecule comprising a targeting sequence that is complementary to at least a portion of the polynucleotide sequence.
A transgenic plant comprising a recombinant polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, wherein expression of the polynucleotide sequence is spatially and temporally restricted by a small RNA molecule.
Dependencies are inferred from the claim text.