Preserving spatial-proximal contiguity and molecular contiguity in nucleic acid templates
AssigneeArima Genomics, Inc.
InventorsSiddarth Selvaraj, Anthony Schmitt, Bret Reid
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Abstract
Provided herein are methods and compositions for preparing nucleic acid templates wherein spatial-proximal and molecular contiguity of target nucleic acids is preserved, and the sequencing data obtained therefrom is used, but not limited to, identification of genomic variants, determination of contiguity information to inform assemblies of target nucleic acids de novo including deconvolution of haplotype phase information, and analyses of conformation and topology of target nucleic acids.
Claims18
1-169. (canceled)
A method for preparing library nucleic acid templates that preserves spatial-proximal and molecular contiguity, comprising:
- reacting isolated nucleic acid with reagents that generate proximity ligated nucleic acid molecules;
- preparing templates from the proximity ligated nucleic acid molecules, wherein sequencing the templates using a sequencer that generates sequence reads of about 2 kilobases or greater.
Dependencies are inferred from the claim text.