Single-component near-infrared optogenetic systems for gene transcription regulation

Abstract

This disclosure provides the single-component near-infrared light-controlled IsPadC-PCM-based optogenetic systems for prokaryotic and eukaryotic cells, consisting of an evolved photosensory core module of the Idiomarina sp. bacterial phytochrome, named iLight. The iLight systems are smaller and packable in an adeno-associated virus, as compared to the other near-infrared optogenetic systems based on phytochromes. This disclosure demonstrates the high light-activation efficiency of the developed iLight systems in gene transcription regulation in bacteria, cultured mammalian cells, primary isolated neurons, and living mouse tissue in vivo. The iLight systems also enable crosstalk-free spectral multiplexing with optogenetic systems and fluorescent probes activated or excited by light of the visible spectral range.

Dependencies are inferred from the claim text.