Trispecific antibody and preparation method therefor and use thereof

A hexavalent trispecific antibody, wherein the hexavalent trispecific antibody is a dimer formed by two monomers, and each monomer comprises a heavy chain, a first light chain and a second light chain;

• the heavy chain comprises an antibody heavy chain variable region element Z1 for a first target, an antibody heavy chain variable region element Z2 for a second target, an antibody heavy chain variable region element Z3 for a third target, and a antibody heavy chain constant region Z4 in tandem from the N end to the C end;
• the first light chain and the second light chain respectively cooperate with the heavy chain, so that the hexavalent trispecific antibody specifically binds to the first target, second target, and third target.