Delivery of card protein as therapy for occular inflammation
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsCristhian J. Ildefonso, Alfred S. Lewin, Qiuhong Li
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Abstract
The present invention provides methods and compositions for treating and/or preventing age related macular degeneration and other conditions involving macular degeneration, ocular neovascularization, or ocular inflammation. In an exemplary embodiment, a method is disclosed that involves administering an expression vector that delivers a secretable and cell penetrating CARD to a subject in need of treatment or prevention of age-related macular degeneration or another condition involving macular degeneration or ocular neovascularization.
Claims19
1-21. (canceled)
A pharmaceutical composition comprising:
- i) a recombinant adeno-associated virus (rAAV) vector comprising an expression construct comprising (a) a nucleotide sequence encoding a fusion protein comprising a cell-penetrating peptide derived from the HIV Tat protein (Tat) which comprises amino acids 1-9 of the amino acid sequence of SEQ ID NO:
- 1 and a Caspase Activation and Recruitment Domain (CARD) which comprises amino acids 10-100 of the amino acid sequence of SEQ ID NO:
- 1, and (b) a nucleotide sequence encoding a secretion signal peptide, wherein the expression construct is flanked by inverted terminal repeat (ITR) regions;
- and ii) a carrier, a diluent and/or an excipient;
- wherein the composition is formulated for delivery into the suprachoroidal space.
A method for the amelioration or treatment of an ocular disease or ocular condition in a subject, the method comprising:
- administering to the suprachoroidal space of the subject's eye a therapeutically effective amount of a recombinant adeno-associated virus (rAAV) vector comprising an expression construct comprising (a) a nucleotide sequence encoding a fusion protein comprising a cell-penetrating peptide derived from the HIV Tat protein (Tat) which comprises amino acids 1-9 of the amino acid sequence of SEQ ID NO:
- 1 and a Caspase Activation and Recruitment Domain (CARD) which comprises amino acids 10-100 of the amino acid sequence of SEQ ID NO:
- 1, and (b) a nucleotide sequence encoding a secretion signal peptide, wherein the expression construct is flanked by inverted terminal repeat (ITR) regions.
A nucleotide sequence encoding a fusion protein comprising (a) a cell-penetrating peptide derived from the HIV Tat protein (Tat) which comprises amino acids 1-9 of the amino acid sequence of SEQ ID NO:
- 1 and a Caspase Activation and Recruitment Domain (CARD) which comprises amino acids 10-100 of the amino acid sequence of SEQ ID NO:
- 1, and (b) a secretion signal peptide.
Dependencies are inferred from the claim text.