Chemical Phosphorylation Reagents, Preparation, and Their Uses

A compound, having the formula:

• or a salt thereof,
• wherein,
• X is H,
• J1 is O, NH, or S;
• J2 is a macromolecule;
• R1 is C1-6 alkyl;
• R2 is C1-6 alkyl;
• L1 is C1-6 alkylene, C1-6 alkenylene, or C1-6 alkynylene;
• R3 is a bond, O, N(H), N(CH3), S, C2-6 alkylene, C2-6 alkenylene, C2-6 alkynylene, SS, O—N═C, C═N—O, C(O)N(H), N(H)C(O), OC(O)O, OC(O)N(H), N(H)C(O)O, N(H)C(O)N(H), OC(O)N(C1-6 alkyl), N(C1-6 alkyl)C(O)O, N(C1-6 alkyl)C(O)N(C1-6 alkyl), C(H)═N, or N═C(H);
• L2 is C1-6 alkylene, C1-6 alkenylene, or C1-6 alkynylene;
• R4 is O or S;
• R5 is H or an electron donating moiety;
• R6 is H or an electron donating moiety;
• and
• R7 is H or an electron donating moiety;
• wherein the compound is not 2-((2-(bis(4-methoxyphenyl)(phenyl)methoxy)ethyl)disulfaneyl)ethyl (2-cyanoethyl) diisopropylphosphoramidite or 2-((2-(bis(4-methoxyphenyl)(phenyl)methoxy)ethyl)disulfaneyl)ethan-1-ol.