Back to results
US 2024/0253045 A1Published application
Microfluidic method for single cell analysis
AssigneeHifibio SAS
InventorsAnnabelle Patricia Veronique Gérard, Vera Menrath
Life of this patent
Estimating the term…
Abstract
The present invention concerns a method for capturing and barcoding nucleic acid from single cells, a plurality of microfluidic droplets and a method for preparing said plurality of microfluidic droplets.
Claims16
A method for the detection of a compound of interest in a microfluidic system, said method comprising:
- a. Creating at least one droplet capable of generating a detectable event, said at least one droplet comprising:
- i. at least one single cell,
- ii. one or more first capturing agent, wherein said one or more first capturing agent is capable of binding said at least one single cell as well as said compound of interest,
- iii. one or more second capturing agent comprising a label, wherein said one or more second capturing agent is capable of binding said compound of interest,
- b. incubating said at least one droplet capable of generating a detectable event,
- c. subjecting said at least one droplet to a direct detection in said microfluidic system,
- wherein the presence or relocalization of said detectable event within said at least one droplet is indicative of the presence of said compound of interest.
4 more elements
A method to monitor a biological event, said method comprising:
- a. creating at least one droplet capable of generating a detectable event, said at least one droplet comprising:
- i. at least one single cell,
- ii. one or more first capturing agent, wherein said one or more first capturing agent is capable of binding said at least one single cell as well as a compound of interest, and
- iii. one or more second capturing agent comprising a label, wherein said one or more second capturing agent is capable of binding said compound of interest,
- b. incubating said at least one droplet, and
- c. subjecting said at least one droplet to a direct detection in said microfluidic system,
- wherein the presence or relocalization of said detectable event within said at least one droplet is indicative of the presence of said compound of interest.
4 more elements
A method for the detection of a compound of interest in a droplet comprising:
- a. providing a microfluidic system comprising:
- i. at least one inlet,
- ii. at least one outlet,
- iii. one or more channels,
- b. injecting in a microfluidic system a stream of droplets, wherein at least one droplet comprises:
- i. at least one single cell,
- ii. a plurality of a first capturing agents capable of binding said at least one single cell as well as said compound of interest, and
- iii. a plurality of second capturing agents, each comprising a label, wherein said plurality of second capturing agents is capable of binding said compound of interest,
- c. incubating said droplets under conditions that allow the production of the compound of interest, whereby if the compound of interest is produced by said at least one single cell, it is capable of being captured by said plurality of first and second capturing agents,
- d. determining the presence of the compound of interest by detecting, in said microfluidic system, a presence or relocalization of said label.
7 more elements
A microfluidic system comprising:
- a. at least one inlet,
- b. at least one outlet,
- c. one or more channels,
- d. a module for creating at least one droplet comprising:
- a. one or more single cell,
- b. a first capturing agent,
- c. a second capturing agent.
- e. a detection module detecting droplet containing cells producing compound of interest, and
- f. an analysis module configured for the analysis of the signal.
6 more elements
Dependencies are inferred from the claim text.