Microfluidic detection system with adjustable flow control

A detection system for detecting an analyte in a sample, comprising:

• a microfluidic chip comprising:
• an inlet adapted to receive the sample,
• an incubation chamber having an incubation chamber inlet fluidly connected to the inlet downstream thereof, to incubate the analyte in the sample,
• a sensing chamber fluidly connected to the incubation chamber, downstream of the filter barrier, and
• an outlet fluidly connected to the sensing chamber downstream thereof;
• a suction membrane in fluid communication with the outlet of the microfluidic chip;
• an actuator for applying or relieving pressure from the suction membrane which modifies the air pressure in the microfluidic chip and drives a flow of the sample in the microfluidic chip;
• and
• a detection apparatus for measuring a signal of the analyte in the sensing chamber.