Neurite outgrowth promotion kit and use therefor
AssigneeKeio University
InventorsHideyuki OKANO, Yoshitaka KASE
Life of this patent
Estimating the term…
Abstract
A neurite outgrowth promotion kit containing a γ-secretase inhibitor or a GADD45G expression vector, in which the neurite outgrowth promotion kit is used for significantly promoting neurite outgrowth of nerve cells as compared with a control, by acting on a neurosphere to induce differentiation of the neurosphere into nerve cells; a method for producing a neurosphere for treatment of spinal cord injury, which includes a step of using the above-described neurite outgrowth promotion kit on a neurosphere derived from a pluripotent stem cell; a neurosphere for treatment of spinal cord injury; and a method for selecting a neurosphere for treatment of spinal cord injury and a method for screening a neurite outgrowth-promoting agent.
Claims10
1-8. (canceled)
A method for producing a neurosphere for treatment of spinal cord injury, comprising:
- using a neurite outgrowth promotion kit on a neurosphere derived from pluripotent stem cells,
- wherein the neurite outgrowth promotion kit comprises a γ-secretase inhibitor or a GADD45G expression vector.
A neurosphere for treatment of spinal cord injury,
- wherein an expression level of GADD45G or an amount of phosphorylated CDC25B is increased as compared with a control.
A method for selecting a neurosphere for treatment of spinal cord injury, the method comprising:
- measuring an expression level of GADD45G or an amount of phosphorylated CDC25B in a neurosphere,
- wherein a case where the measured expression level of the GADD45G or the measured amount of the phosphorylated CDC25B is increased as compared with a control indicates that the neurosphere is suitable for treatment of spinal cord injury.
A method for screening a neurite outgrowth-promoting agent, comprising:
- culturing a neurosphere in a presence of a test substance;
- and
- measuring an expression level of GADD45G or an amount of phosphorylated CDC25B in the neurosphere,
- wherein a case where the expression level of the GADD45G or the amount of the phosphorylated CDC25B is increased as compared with a case where the test substance is not present indicates that the test substance is a neurite outgrowth-promoting agent.
Dependencies are inferred from the claim text.