Methods for Making Stem Cell-Derived Enteric Neural Crest Cells and Their Use in Enteric Neuropathy Treatment
AssigneeChildren's Hospital Los Angeles
InventorsTracy Grikscheit, Laura-Marie Nucho
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Abstract
Provided herein are compositions and methods to treat enteric neuropathies/or slow gut motility comprising intra-muscularly administering enteric neural crest cells (ENCCs) to a intestine of a subject in need thereof, wherein the ENCCs are differentiated from stem cells in vitro.
Claims38
A method to treat an enteric neuropathy comprising administering enteric neural crest cells (ENCCs) to a muscular wall of the intestine in a subject in need thereof, wherein the ENCCs are differentiated from stem cells in vitro.
A composition comprising enteric neural crest cells (ENCCs) differentiated from stem cells in vitro and a cryopreservative.
A neurosphere comprising a heterogeneous mixture of cells, wherein the mixture of cells are positive for expression HNK-1, positive for expression p75, negative for expression Oct4 and negative for expression Nanog, wherein the expression of HNK-1, p75, Oct4 and/or Nanog is from one cell or each marker is expressed individually or in combination on different cells.
A method for producing enteric neural crest cells (ENCCs) comprising the steps of:
- a) expanding stem cells in suspension;
- b) contacting the stem cells of a) with at least one inhibitor of SMAD, at least one activator of the WNT pathway, FGF2 and retinoic acid and culturing said stem cells for a period of time and under conditions sufficient to differentiate said stem cells to enteric neural crest cells (ENCCs);
- and
- c) isolating said enteric neural crest cells (ENCCs) of b).
Dependencies are inferred from the claim text.