Method for Producing Retinal Tissue
AssigneesRiken, Sumitomo Pharma Co., Ltd.
InventorsMichiko Mandai, Suguru Yamasaki
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Abstract
An object of the present invention is to provide a method for producing a retinal cell or retinal tissue with a reduced proportion of non-target cells in differentiating pluripotent stem cells. The method of the present invention for producing a retinal cell or retinal tissue comprises: (A) suspension-culturing a pluripotent stem cell to form a cell aggregate of pluripotent stem cells; and (B) suspension-culturing the cell aggregate obtained in the step (A) in the presence of a BMP signaling pathway agonist and a CHK1 signaling pathway inhibitor to obtain a cell aggregate containing a retinal cell.
Claims18
A method for producing a retinal cell or retinal tissue, comprising:
- (A) suspension-culturing a pluripotent stem cell to form a cell aggregate of pluripotent stem cells;
- and
- (B) suspension-culturing the cell aggregate obtained in the step (A) in the presence of a BMP signaling pathway agonist and a CHK1 signaling pathway inhibitor to obtain a cell aggregate containing a retinal cell.
A sphere-like cell aggregate comprising:
- a multilayer structure of an outer structure including a retinal pigment epithelial cell and an inner structure including a neural retina, wherein
- (1) a neural retinal layer including at least a photoreceptor layer is formed in the neural retina in the inner structure, and the photoreceptor layer includes one or more cells selected from the group consisting of a photoreceptor cell, a photoreceptor progenitor cell and a retinal progenitor cell,
- (2) the neural retina is present in a folded state in the inner structure,
- (3) the retinal pigment epithelial cell in the outer structure is an RPE65-positive cell, an MITF-positive cell, or an RPE65-positive and MITF-positive cell, and
- (4) the cell aggregate is free of a lens, a vitreous body, a cornea and a blood vessel.
Dependencies are inferred from the claim text.