Exosome, preparation method thereof, use thereof and pharmaceutical composition
AssigneeChina Medical University
InventorsLong-Bin Jeng, Mien-Chie Hung, Woei-Cherng Shyu, Syuan-Ling Lin, Chien-Lin Chen
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Abstract
An exosome, a preparation method of an exosome, uses of the exosome, and a pharmaceutical composition including the exosome for treating an ischemia condition of a tissue are provided. The exosome is derived from a genetically engineered mesenchymal stem cell, and the genetically engineered mesenchymal stem cell includes an exogenous PD-L1 gene and an exogenous HGF gene.
Claims23
An exosome derived from a genetically engineered mesenchymal stem cell, wherein the genetically engineered mesenchymal stem cell comprises an exogenous PD-L1 gene and an exogenous HGF gene.
A preparation method of an exosome, comprising:
- constructing a genetically engineered mesenchymal stem cell, wherein an exogenous HGF gene and an exogenous PD-L1 gene are transferred into a mesenchymal stem cell to obtain the genetically engineered mesenchymal stem cell;
- performing a culture step, wherein the genetically engineered mesenchymal stem cell is cultured in a culture medium to obtain a conditioned medium;
- and
- performing an isolation step, wherein an exosome is collected from the conditioned medium by an isolating method.
Dependencies are inferred from the claim text.