# US 2024/0248293 A1 · Microscope illuminator

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2024/0248293 A1
- Document ID: US20240248293A1
- Status: Published application
- Kind: A1
- Application number: 18/408,428
- Filed: 2024-01-09
- Published: 2024-07-25
- Earliest claimed: 2023-01-23
- Assignees: Mikroscan Technologies
- Applicants: Mikroscan Technologies
- Inventors: Jeffrey Ross Gray
- Web page: https://patentgrep.com/patent/US20240248293A1

## CPC

- G02B 21/06: Means for illuminating specimens
- G02B 21/086: {for transillumination only}
- G02B 21/0032: {Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers (G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06)}
- G02B 21/0076: {arrangements using fluorescence or luminescence}
- G02B 21/28: with cooling device
- G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS

## Abstract

A microscope is disclosed that has a base, a slide holder configured to accept a slide, and an illuminator comprising a light-emitting surface configured to project light on an optical path toward the slide when the slide is accepted by the slide holder. There is no light-shaping apparatus disposed on the optical path between the illuminator and the slide holder.

## Claims

### Claim 1

A microscope, comprising:
a base;
a slide holder coupled to the base and configured to accept a slide; and
an illuminator movably coupled to the base and comprising a light-emitting surface configured to project light on an optical path toward the slide when the slide is accepted by the slide holder;
wherein:
there is no light-shaping optical element apparatus disposed on the optical path between the illuminator and the slide holder.

### Claim 2 (depends on 1)

The microscope of claim 1, wherein the illuminator can be moved along the optical path with a separation distance between the light-emitting surface and the slide.

### Claim 3 (depends on 2)

The microscope of claim 2, further comprising:
an objective coupled to the base and disposed so as to observe a portion of the slide illuminated by the projected light;
wherein:
the objective has an aperture and an acceptance angle;
the projected light reaches the aperture of the objective within an arrival angle that is inversely related to the separation distance;
increasing the separation distance reduces the arrival angle of the projected light that enters the aperture of the objective, thereby reducing a realized numerical aperture of the microscope.

### Claim 4 (depends on 2)

The microscope of claim 2, wherein:
the illuminator has an adjustable intensity; and
the intensity is adjusted automatically based in part on the separation distance.

### Claim 5 (depends on 4)

The microscope of claim 4, wherein the intensity is adjusted automatically to maintain a selected brightness as the separation distance is changed.

### Claim 6 (depends on 1)

The microscope of claim 1, wherein the illuminator comprises a light emitting diode (LED).

### Claim 7 (depends on 6)

The microscope of claim 6, wherein:
the objective has a Field of View (FOV) with a first smallest dimension;
the light-emitting surface has a second smallest dimension;
the projected light has a peak intensity on an axis perpendicular to the light-emitting surface and a 90% intensity at a determined half-angle from the perpendicular axis;
the projected light has an intensity over the entire FOV that is within a range of 10% of the peak intensity when the separation distance is at least the arcsine of the half-angle.

### Claim 8 (depends on 7)

The microscope of claim 7, wherein changing the separation distance changes the realized numerical aperture of the microscope.

### Claim 9 (depends on 1)

The microscope of claim 1, wherein the projected light is essentially grainless.

### Claim 10

An illuminator for use with a microscope having a base, a slide holder configured to accept a slide, and an objective having a Field Of View (FOV) covering a portion of an accepted slide, the illuminator comprising a light-emitting surface and configured to be movably coupled to the base, wherein a clear and focused image can be provided by the objective with no light-shaping optical element disposed along an optical path from the illuminator to the accepted slide.

### Claim 11 (depends on 10)

The illuminator of claim 10, wherein:
the illuminator is movable with respect to the base to selectably determine a separation distance between the light-emitting surface and the slide;
the light-emitting surface configured to project light on the optical path;
the projected light has a peak intensity on an axis perpendicular to the light-emitting surface and a 90% intensity at a determined first half-angle from the perpendicular axis;
the projected light has an intensity over the entire FOV that is within a range of 10% of the peak intensity when the separation distance is at least the arcsine of the half-angle.

### Claim 12 (depends on 11)

The illuminator of claim 11, wherein changing the separation distance changes the realized numerical aperture of the microscope.

### Claim 13

A method of operating a microscope, comprising:
projecting light from a planar light-emitting surface directly onto an underside of a slide accepted by the microscope; and
adjusting a separation distance between the light-emitting surface and the underside of the slide to achieve a selected value of a realized numerical aperture of the microscope.

### Claim 14 (depends on 13)

The method of claim 13, wherein:
the light emitted by the light-emitting surface has an intensity that is uniform within a defined range over a dispersion cone having a defined half-angle;
the projected light is not shaped between the light-emitting surface and the underside of the slide; and
increasing the separation distance reduces a maximum half-angle of the emitted light that enters an entrance aperture of an objective of the microscope.

### Claim 15 (depends on 14)

The method of claim 14, wherein:
the light-emitting surface comprises a light emitting diode (LED); and
reduces the maximum half-angle of the emitted light that enters the entrance aperture of the objective reduces the realized numerical aperture of the microscope.
